Alpha-amanitin blocks translocation by human RNA polymerase II
- PMID: 15096519
- DOI: 10.1074/jbc.M402163200
Alpha-amanitin blocks translocation by human RNA polymerase II
Abstract
Our laboratory has developed methods for transient state kinetic analysis of human RNA polymerase II elongation. In these studies, multiple conformations of the RNA polymerase II elongation complex were revealed by their distinct elongation potential and differing dependence on nucleoside triphosphate substrate. Among these are conformations that appear to correspond to different translocation states of the DNA template and RNA-DNA hybrid. Using alpha-amanitin as a dynamic probe of the RNA polymerase II mechanism, we show that the most highly poised conformation of the elongation complex, which we interpreted previously as the posttranslocated state, is selectively resistant to inhibition with alpha-amanitin. Because initially resistant elongation complexes form only a single phosphodiester bond before being rendered inactive in the following bond addition cycle, alpha-amanitin inhibits elongation at each translocation step.
Similar articles
-
Formation of a single phosphodiester bond by RNA polymerase B from calf thymus is not inhibited by alpha-amanitin.Biochemistry. 1982 Jun 22;21(13):3097-101. doi: 10.1021/bi00256a010. Biochemistry. 1982. PMID: 7104312
-
Studies on the inhibition by alpha-amanitin of single-step addition reactions and productive RNA synthesis catalysed by wheat-germ RNA polymerase II.Biochem J. 1989 Feb 15;258(1):165-9. doi: 10.1042/bj2580165. Biochem J. 1989. PMID: 2467661 Free PMC article.
-
Formation of transcription preinitiation complexes with an amanitin-resistant RNA polymerase II.J Biol Chem. 1988 Nov 15;263(32):17128-35. J Biol Chem. 1988. PMID: 3182839
-
Giardia lamblia RNA polymerase II: amanitin-resistant transcription.J Biol Chem. 2003 Jul 25;278(30):27804-10. doi: 10.1074/jbc.M303316200. Epub 2003 May 6. J Biol Chem. 2003. PMID: 12734189
-
Transcription initiation by RNA polymerase II in vitro. At least two nucleotides must be added to form a stable ternary complex.J Biol Chem. 1987 Jan 5;262(1):289-97. J Biol Chem. 1987. PMID: 2432060
Cited by
-
Chromatin structure analyses identify miRNA promoters.Genes Dev. 2008 Nov 15;22(22):3172-83. doi: 10.1101/gad.1706508. Genes Dev. 2008. PMID: 19056895 Free PMC article.
-
Structural perspective on mutations affecting the function of multisubunit RNA polymerases.Microbiol Mol Biol Rev. 2006 Mar;70(1):12-36. doi: 10.1128/MMBR.70.1.12-36.2006. Microbiol Mol Biol Rev. 2006. PMID: 16524917 Free PMC article. Review.
-
De novo mRNA synthesis is required for both consolidation and reconsolidation of fear memories in the amygdala.Learn Mem. 2008 Oct 2;15(10):747-55. doi: 10.1101/lm.1027208. Print 2008 Oct. Learn Mem. 2008. PMID: 18832561 Free PMC article.
-
Recruitment and retention dynamics of RECQL5 at DNA double strand break sites.DNA Repair (Amst). 2012 Jul 1;11(7):624-35. doi: 10.1016/j.dnarep.2012.05.001. Epub 2012 May 24. DNA Repair (Amst). 2012. PMID: 22633600 Free PMC article.
-
Transient-state kinetic analysis of multi-nucleotide addition catalyzed by RNA polymerase I.Biophys J. 2021 Oct 19;120(20):4378-4390. doi: 10.1016/j.bpj.2021.09.008. Epub 2021 Sep 10. Biophys J. 2021. PMID: 34509510 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
