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. 2004 Mar;78(6):3196-9.
doi: 10.1128/jvi.78.6.3196-3199.2004.

Complete protection from papillomavirus challenge after a single vaccination with a vesicular stomatitis virus vector expressing high levels of L1 protein

Anjeanette Roberts  1 , Jon D Reuter, Jean H Wilson, Stuart Baldwin, John K Rose
Affiliations

Complete protection from papillomavirus challenge after a single vaccination with a vesicular stomatitis virus vector expressing high levels of L1 protein

Anjeanette Roberts et al. J Virol. 2004 Mar.

Abstract

We generated an attenuated, recombinant vesicular stomatitis virus (VSV) expressing high levels of the cottontail rabbit papillomavirus (CRPV) L1 protein from an upstream site in the VSV genome. Rabbits vaccinated once with this VSV-L1 recombinant produced high levels of anti-L1 antibody and were completely protected against papilloma formation after challenge with CRPV. In contrast, animals vaccinated only once with a VSV vector expressing lower levels of L1 from a downstream site in the VSV genome generated lower levels of L1 antibody and demonstrated only incomplete protection from papilloma formation after challenge. We conclude that the level of L1 protein expression is critical in generating complete immunity with a single-dose vaccine.

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Figures

FIG. 1.
FIG. 1.
Diagram of VSV-L1 recombinants and relative expression of L1 proteins. (A) Schematic representation of gene order in wild-type VSV and in the two recombinant VSVs expressing CRPV-L1 proteins. Gene order is presented in the 3′-to-5′ orientation on the negative-strand genome. (B) Metabolically labeled proteins from crude lysates of BHK cells infected with the indicated viruses (lanes 1 to 3) or immunoprecipitates of the same lysates with a rabbit polyclonal antibody to L1 protein (lanes 4 to 6).
FIG. 2.
FIG. 2.
Protection from CRPV challenge with VSVL1-2 and VSVL1-5 vectors. (A) Average percentages of papilloma-free sites for rabbits immunized with a single dose of VSVL1-2 or VSVL1-5 vaccine or for unvaccinated controls. The error bars represent standard errors of the means. (B and C) Average high-dose (B) and low-dose (C) papilloma volumes (natural log) following vaccination. Filled circle, VSVL1-5; ✻, VSVL1-2; diamond, unvaccinated control.
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