Comparative Study
Nonradioactive southwestern analysis using chemiluminescent detection
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- PMID: 1476722
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Comparative Study
Nonradioactive southwestern analysis using chemiluminescent detection
Biotechniques.
1992 Oct.
Abstract
Replacing radioactively labeled probes by nonradioactive ones and detection by chemiluminescence instead of colorimetry allows a nonhazardous handling and offers the possibility of easily reprobing filters in Southwestern analysis. Using the described procedure, we were able to determine the molecular weight of DNA-binding proteins and achieve a high signal-to-noise ratio.
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