Primer pair p289-p290, designed to detect both noroviruses and sapoviruses by reverse transcription-PCR, also detects rotaviruses by cross-reactivity

doi:10.1128/JCM.42.2.835-836.2004

. 2004 Feb;42(2):835-6.

doi: 10.1128/JCM.42.2.835-836.2004.

Primer pair p289-p290, designed to detect both noroviruses and sapoviruses by reverse transcription-PCR, also detects rotaviruses by cross-reactivity

Juan E Ludert¹, Ana C Alcalá, Ferdinando Liprandi

Affiliations

PMID: 14766865
PMCID: PMC344525
DOI: 10.1128/JCM.42.2.835-836.2004

Primer pair p289-p290, designed to detect both noroviruses and sapoviruses by reverse transcription-PCR, also detects rotaviruses by cross-reactivity

Juan E Ludert et al. J Clin Microbiol. 2004 Feb.

. 2004 Feb;42(2):835-6.

doi: 10.1128/JCM.42.2.835-836.2004.

Authors

Juan E Ludert¹, Ana C Alcalá, Ferdinando Liprandi

Affiliation

¹ Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela. jeludert@ivic.ve

PMID: 14766865
PMCID: PMC344525
DOI: 10.1128/JCM.42.2.835-836.2004

Abstract

A primer pair (p289-p290) designed to detect both noroviruses and sapoviruses by reverse transcription-PCR (Jiang et al., J. Virol. Methods 83:145, 1999) cross-reacts with rotaviruses. The rotavirus amplicon corresponds to genome segment 1. Furthermore, primer pair p289-p290 detected rotaviruses as efficiently as rotavirus-specific primers directed to rotavirus gene 4.

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References

1. Abbaszadegan, M., P. Steward, and M. LeChevalier. 1999. A strategy for detection of viruses in groundwater by PCR. Appl. Environ. Microbiol. 65:444-449. - PMC - PubMed
1. Atmar, R. L., and M. K. Estes. 2001. Diagnosis of noncultivable gastrointestinal viruses, the human calicivirus. Clin. Microbiol. Rev. 14:15-37. - PMC - PubMed
1. Green K. Y., R. M. Chanock, and A. Z. Kapikian. 2001. Human caliciviruses, p. 841-874. In D. M. Knipe, P. M. Howley, D. E. Griffin, R. A. Lamb, M. A. Martin, B. Roizman, and S. E. Straus (ed.), Fields' virology, 4th ed. Lippincott Williams & Wilkins, Philadelphia, Pa.
1. Green, S. M., P. R. Lambden, Y. Deng, A. Lowes, S. Lineham, J. Bushell, J. Rogers, E. O. Caul, C. R. Ashley, and I. N. Clarke. 1995. Polymerase chain reaction detection of small round-structured viruses from two related hospital outbreaks of gastroenteritis using inosine-containing primers. J. Med. Virol. 45:197-202. - PubMed
1. Jiang, X., P. W. Huang, W. M. Zhong, T. Farkas, D. W. Cubitt, and D. O. Matson. 1999. Design and evaluation of a primer pair that detects both Norwalk- and Sapporo-like caliciviruses by RT-PCR. J. Virol. Methods 83:145-154. - PubMed

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[1] Abbaszadegan, M., P. Steward, and M. LeChevalier. 1999. A strategy for detection of viruses in groundwater by PCR. Appl. Environ. Microbiol. 65:444-449. - PMC - PubMed

[2] Abbaszadegan, M., P. Steward, and M. LeChevalier. 1999. A strategy for detection of viruses in groundwater by PCR. Appl. Environ. Microbiol. 65:444-449. - PMC - PubMed

[3] Atmar, R. L., and M. K. Estes. 2001. Diagnosis of noncultivable gastrointestinal viruses, the human calicivirus. Clin. Microbiol. Rev. 14:15-37. - PMC - PubMed

[4] Atmar, R. L., and M. K. Estes. 2001. Diagnosis of noncultivable gastrointestinal viruses, the human calicivirus. Clin. Microbiol. Rev. 14:15-37. - PMC - PubMed

[5] Green K. Y., R. M. Chanock, and A. Z. Kapikian. 2001. Human caliciviruses, p. 841-874. In D. M. Knipe, P. M. Howley, D. E. Griffin, R. A. Lamb, M. A. Martin, B. Roizman, and S. E. Straus (ed.), Fields' virology, 4th ed. Lippincott Williams & Wilkins, Philadelphia, Pa.

[6] Green K. Y., R. M. Chanock, and A. Z. Kapikian. 2001. Human caliciviruses, p. 841-874. In D. M. Knipe, P. M. Howley, D. E. Griffin, R. A. Lamb, M. A. Martin, B. Roizman, and S. E. Straus (ed.), Fields' virology, 4th ed. Lippincott Williams & Wilkins, Philadelphia, Pa.

[7] Green, S. M., P. R. Lambden, Y. Deng, A. Lowes, S. Lineham, J. Bushell, J. Rogers, E. O. Caul, C. R. Ashley, and I. N. Clarke. 1995. Polymerase chain reaction detection of small round-structured viruses from two related hospital outbreaks of gastroenteritis using inosine-containing primers. J. Med. Virol. 45:197-202. - PubMed

[8] Green, S. M., P. R. Lambden, Y. Deng, A. Lowes, S. Lineham, J. Bushell, J. Rogers, E. O. Caul, C. R. Ashley, and I. N. Clarke. 1995. Polymerase chain reaction detection of small round-structured viruses from two related hospital outbreaks of gastroenteritis using inosine-containing primers. J. Med. Virol. 45:197-202. - PubMed

[9] Jiang, X., P. W. Huang, W. M. Zhong, T. Farkas, D. W. Cubitt, and D. O. Matson. 1999. Design and evaluation of a primer pair that detects both Norwalk- and Sapporo-like caliciviruses by RT-PCR. J. Virol. Methods 83:145-154. - PubMed

[10] Jiang, X., P. W. Huang, W. M. Zhong, T. Farkas, D. W. Cubitt, and D. O. Matson. 1999. Design and evaluation of a primer pair that detects both Norwalk- and Sapporo-like caliciviruses by RT-PCR. J. Virol. Methods 83:145-154. - PubMed

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Primer pair p289-p290, designed to detect both noroviruses and sapoviruses by reverse transcription-PCR, also detects rotaviruses by cross-reactivity

Affiliation

Primer pair p289-p290, designed to detect both noroviruses and sapoviruses by reverse transcription-PCR, also detects rotaviruses by cross-reactivity

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