Antibody-dependent cellular cytotoxicity via humoral immune epitope of Nef protein expressed on cell surface
- PMID: 14764710
- DOI: 10.4049/jimmunol.172.4.2401
Antibody-dependent cellular cytotoxicity via humoral immune epitope of Nef protein expressed on cell surface
Abstract
Antibodies against various proteins of HIV type 1 (HIV-1) can be detected in HIV-1-infected individuals. We previously reported that the level of Ab response against one Nef epitope is correlated with HIV-1 disease progression. To elucidate the mechanism for this correlation, we examined Ab-dependent cellular cytotoxicity (ADCC) against target cells expressing Nef. We observed efficient cytotoxicity against Nef-expressing target cells in the presence of patient plasma and PBMCs. This ADCC activity was correlated with the dilution of plasma from HIV-1-infected patients. Addition of a specific synthetic peptide (peptide 31:FLKEKGGLE) corresponding to the Nef epitope reduced cell lysis to approximately 50%. These results suggest that PBMCs of HIV-1-infected patients may exert ADCC via anti-Nef Abs in the patients' own plasma and serve as a mechanism used by the immune system to regulate HIV-1 replication.
Similar articles
-
Incomplete Downregulation of CD4 Expression Affects HIV-1 Env Conformation and Antibody-Dependent Cellular Cytotoxicity Responses.J Virol. 2018 Jun 13;92(13):e00484-18. doi: 10.1128/JVI.00484-18. Print 2018 Jul 1. J Virol. 2018. PMID: 29669829 Free PMC article.
-
Nef Proteins from HIV-1 Elite Controllers Are Inefficient at Preventing Antibody-Dependent Cellular Cytotoxicity.J Virol. 2015 Dec 30;90(6):2993-3002. doi: 10.1128/JVI.02973-15. J Virol. 2015. PMID: 26719277 Free PMC article.
-
Expression of a novel Nef epitope on the surface of HIV type 1-infected cells.AIDS Res Hum Retroviruses. 1999 Jul 20;15(11):1001-9. doi: 10.1089/088922299310511. AIDS Res Hum Retroviruses. 1999. PMID: 10445812
-
Current approaches to vaccination against human immunodeficiency viruses.Allergol Immunopathol (Madr). 1991 Jan-Feb;19(1):11-3. Allergol Immunopathol (Madr). 1991. PMID: 1719790 Review. No abstract available.
-
Unlocking HIV-1 Env: implications for antibody attack.AIDS Res Ther. 2017 Sep 12;14(1):42. doi: 10.1186/s12981-017-0168-5. AIDS Res Ther. 2017. PMID: 28893275 Free PMC article. Review.
Cited by
-
Epitope specificity of human immunodeficiency virus-1 antibody dependent cellular cytotoxicity [ADCC] responses.Curr HIV Res. 2013 Jul;11(5):378-87. doi: 10.2174/1570162x113116660059. Curr HIV Res. 2013. PMID: 24191939 Free PMC article. Review.
-
Antibody-Dependent Cellular Cytotoxicity and NK Cell-Driven Immune Escape in HIV Infection: Implications for HIV Vaccine Development.Adv Virol. 2012;2012:637208. doi: 10.1155/2012/637208. Epub 2012 Apr 29. Adv Virol. 2012. PMID: 22611395 Free PMC article.
-
Enhancing natural killer cell function with gp41-targeting bispecific antibodies to combat HIV infection.AIDS. 2020 Jul 15;34(9):1313-1323. doi: 10.1097/QAD.0000000000002543. AIDS. 2020. PMID: 32287071 Free PMC article.
-
Non-neutralizing epitopes induce robust hepatitis C virus (HCV)-specific antibody-dependent CD56+ natural killer cell responses in chronic HCV-infected patients.Clin Exp Immunol. 2017 Jul;189(1):92-102. doi: 10.1111/cei.12962. Epub 2017 Apr 7. Clin Exp Immunol. 2017. PMID: 28317093 Free PMC article.
-
Antibody-dependent cell-mediated viral inhibition emerges after simian immunodeficiency virus SIVmac251 infection of rhesus monkeys coincident with gp140-binding antibodies and is effective against neutralization-resistant viruses.J Virol. 2011 Jun;85(11):5465-75. doi: 10.1128/JVI.00313-11. Epub 2011 Mar 30. J Virol. 2011. PMID: 21450829 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
