Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004 Jan 30;303(5658):669-72.
doi: 10.1126/science.1092653.

Heterochromatic silencing and HP1 localization in Drosophila are dependent on the RNAi machinery

Manika Pal-Bhadra  1 , Boris A Leibovitch, Sumit G Gandhi, Madhusudana Rao Chikka, Utpal Bhadra, James A Birchler, Sarah C R Elgin
Affiliations

Heterochromatic silencing and HP1 localization in Drosophila are dependent on the RNAi machinery

Manika Pal-Bhadra et al. Science. .

Erratum in

  • Science. 2013 May 24;340(6135):924. Rao, Madhusudana [corrected to Chikka, Madhusudana Rao]

Abstract

Genes normally resident in euchromatic domains are silenced when packaged into heterochromatin, as exemplified in Drosophila melanogaster by position effect variegation (PEV). Loss-of-function mutations resulting in suppression of PEV have identified critical components of heterochromatin, including proteins HP1, HP2, and histone H3 lysine 9 methyltransferase. Here, we demonstrate that this silencing is dependent on the RNA interference machinery, using tandem mini-white arrays and white transgenes in heterochromatin to show loss of silencing as a result of mutations in piwi, aubergine, or spindle-E (homeless), which encode RNAi components. These mutations result in reduction of H3 Lys9 methylation and delocalization of HP1 and HP2, most dramatically in spindle-E mutants.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

Publication types

MeSH terms