doi: 10.1271/bbb.67.2674.
A transient RNA interference assay system using Arabidopsis protoplasts
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- PMID: 14730153
- DOI: 10.1271/bbb.67.2674
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A transient RNA interference assay system using Arabidopsis protoplasts
Biosci Biotechnol Biochem.
2003 Dec.
Free article
Abstract
Double-stranded RNA (dsRNA) induces sequence-specific gene silencing in eukaryotes through a process known as RNA interference (RNAi). RNAi is now used as a powerful tool for functional genomics in many eukaryotes, including plants. We herein report a dsRNA-mediated transient RNAi assay system using protoplasts from Arabidopsis mesophyll cells and suspension-cultured cells (cell line T87). Introduction of dsRNA into protoplasts led to marked silencing of target transgenes. Our assay system would provide a convenient and efficient way to induce RNAi in protoplasts of the model plant Arabidopsis thaliana.
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