Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004 Jan;48(1):348-51.
doi: 10.1128/AAC.48.1.348-351.2004.

Chromosome-encoded ambler class D beta-lactamase of Shewanella oneidensis as a progenitor of carbapenem-hydrolyzing oxacillinase

Laurent Poirel  1 , Claire Héritier, Patrice Nordmann
Affiliations

Chromosome-encoded ambler class D beta-lactamase of Shewanella oneidensis as a progenitor of carbapenem-hydrolyzing oxacillinase

Laurent Poirel et al. Antimicrob Agents Chemother. 2004 Jan.

Abstract

A chromosome-encoded beta-lactamase gene from a Shewanella oneidensis reference strain was cloned and expressed in Escherichia coli. It encoded a carbapenem-hydrolyzing Ambler class D beta-lactamase, OXA-54, that shared 92% amino acid identity with the plasmid-encoded carbapenem-hydrolyzing oxacillinase OXA-48 from Klebsiella pneumoniae. This work suggests that Shewanella spp. may produce the progenitor of oxacillinases compromising the efficacy of imipenem in clinically relevant gram-negative pathogens.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Comparison of the amino acid sequence of the carbapenem-hydrolyzing oxacillinase β-lactamase OXA-54 of reference strain S. oneidensis MR-1 to that of OXA-48 of the K. pneumoniae clinical isolate 11978. Shaded amino acid residues are conserved regions in the oxacillinase family. Numbering of β-lactamases is according to DBL (5). The vertical arrow indicates the putative cleavage site of the leader peptide of the mature β-lactamases.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Afzal-Shah, M., N. Woodford, and D. M. Livermore. 2001. Characterization of OXA-25, OXA-26, and OXA-27, molecular class D β-lactamases associated with carbapenem resistance in clinical isolates of Acinetobacter baumannii. Antimicrob. Agents Chemother. 45:583-588. - PMC - PubMed
    1. Aubert, D., L. Poirel, J. Chevalier, S. Léotard, J.-M. Pagès, and P. Nordmann. 2001. Oxacillinase-mediated resistance to cefepime and susceptibility to ceftazidime in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 45:1615-1620. - PMC - PubMed
    1. Bonnet, R., H. Marchandin, C. Chanal, D. Sirot, R. Labia, C. De Champs, E. Jumas-Bilak, and J. Sirot. 2002. Chromosome-encoded class D β-lactamase OXA-23 in Proteus mirabilis. Antimicrob. Agents Chemother. 46:2004-2006. - PMC - PubMed
    1. Bou, G., A. Oliver, and J. Martinez-Beltran. 2000. OXA-24, a novel class D β-lactamase with carbapenemase activity in an Acinetobacter baumannii clinical strain. Antimicrob. Agents Chemother. 44:1556-1561. - PMC - PubMed
    1. Couture, F., J. Lachapelle, and R. C. Lévesque. 1992. Phylogeny of LCR-1 and OXA-5 with class A and class D β-lactamases. Mol. Microbiol. 6:1693-1705. - PubMed

Publication types

MeSH terms

LinkOut - more resources