doi: 10.1016/j.bmcl.2003.09.067.
Synthesis of hydrophilic and flexible linkers for peptide derivatization in solid phase
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- PMID: 14684320
- DOI: 10.1016/j.bmcl.2003.09.067
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Synthesis of hydrophilic and flexible linkers for peptide derivatization in solid phase
Bioorg Med Chem Lett.
.
Abstract
Four N-Fmoc protected polyoxyethylene-based amino acid type linkers were designed and synthesized for peptide derivatization in solid phase. Three of them were obtained in a crystalline form. The crystallized linkers can be stored at 4 degrees C for 2 years without significant decomposition. Protocols for biotinylation and fluorescent labeling of peptides in solid phase were developed. The linkers also provide good ionization ability for single-bead mass spectrometry analysis of peptides.
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