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. 2004 Mar 5;279(10):9565-76.
doi: 10.1074/jbc.M310739200. Epub 2003 Dec 15.

Focal adhesion kinase regulation of N-WASP subcellular localization and function

Xiaoyang Wu  1 , Shiro Suetsugu, Lee Ann Cooper, Tadaomi Takenawa, Jun-Lin Guan
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Free article

Focal adhesion kinase regulation of N-WASP subcellular localization and function

Xiaoyang Wu et al. J Biol Chem. .
Free article

Abstract

N-WASP is a member of the WASP family of proteins that regulate actin cytoskeleton remodeling. FAK is a cytoplasmic tyrosine kinase implicated in integrin signaling during cell migration. Here we identify a direct interaction between N-WASP and FAK and show that N-WASP is phosphorylated by FAK at a conserved tyrosine residue, Tyr(256). We found that phosphorylation of Tyr(256) affected N-WASP nuclear localization, suggesting that phosphorylation of N-WASP by FAK may regulate its activity in vivo by altering its subcellular localization. We also showed that the nuclear localization of N-WASP is dependent on its being in the open conformation either after its activation by Cdc42 or the truncation of the C-terminal VCA domain. Phosphorylation of Tyr(256) of N-WASP could reduce its interaction with nuclear importin NPI-1, which might be responsible for its decreased nuclear localization. Lastly, we show that phosphorylation of Tyr(256) plays an important role in promoting cell migration. Together, these results suggest a novel regulatory mechanism of N-WASP by tyrosine phosphorylation and subcellular localization and its potential role in the regulation of cell migration.

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