Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2003 Dec 6;362(9399):1895-6.
doi: 10.1016/S0140-6736(03)14962-8.

Effects of a SARS-associated coronavirus vaccine in monkeys

Wentao Gao  1 , Azaibi Tamin, Adam Soloff, Leonardo D'Aiuto, Edward Nwanegbo, Paul D Robbins, William J Bellini, Simon Barratt-Boyes, Andrea Gambotto
Affiliations

Effects of a SARS-associated coronavirus vaccine in monkeys

Wentao Gao et al. Lancet. .

Abstract

The causative agent of severe acute respiratory syndrome (SARS) has been identified as a new type of coronavirus. Here, we have investigated the ability of adenoviral delivery of codon-optimised SARS-CoV strain Urbani structural antigens spike protein S1 fragment, membrane protein, and nucleocapsid protein to induce virus-specific broad immunity in rhesus macaques. We immunised rhesus macaques intramuscularly with a combination of the three Ad5-SARS-CoV vectors or a control vector and gave a booster vaccination on day 28. The vaccinated animals all had antibody responses against spike protein S1 fragment and T-cell responses against the nucleocapsid protein. All vaccinated animals showed strong neutralising antibody responses to SARS-CoV infection in vitro. These results show that an adenoviral-based vaccine can induce strong SARS-CoV-specific immune responses in the monkey, and hold promise for development of a protective vaccine against the SARS causal agent.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Western blot analysis of immunised (1699, 1201, 1401, 11300, 13601 and 15101) and control (1901 and 2001) monkeys Bands indicate presence of S1-specific antibodies in serum.
Figure 2
Figure 2
Nucleocapsid-specific T-cell responses (upper) and neutralising capacity (lower) of serum samples after vaccination Immunised animals represented by black symbols, controls by white symbols. (Upper) ELISPOT analysis 24 h after monkey peripheral blood mononuclear cells (PBMC) were incubated with pools of nucleocapsid-specific peptides and interferon-γ (IFN-γ) spot-forming cells (SFC) were counted. Data are mean (SE) of triplicate determinations after subtraction of background responses. (Lower) Detection of virus-specific neutralising antibodies in serum by inhibition of E6 Vero cell lysis by SARS-CoV strain Urbani in a microneutralisation assay. Data for 1699 and 1201 overlap at all time points.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Rota PA, Oberste MS, Monroe SS. Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science. 2003;300:1394–1399. - PubMed
    1. Anton IM, Gonzalez S, Bullido MJ. Cooperation between transmissible gastroenteritis coronavirus (TGEV) structural proteins in the in vitro induction of virus-specific antibodies. Virus Res. 1996;46:111–124. - PMC - PubMed
    1. Brown K, Gao W, Alber S, etal. Adenovirus-transduced dendritic cells injected into skin or lymph node prime potent siv-specific t-cell immunity in monkeys. J Immunol (in press). - PubMed
    1. Weiss RC, Scott W. Antibody-mediated enhancement of disease in feline infectious peritonitis: comparisons with dengue hemorrhagic fever. Comp Immunol Microbiol Infect Dis. 1981;4:175–189. - PMC - PubMed
    1. Fouchier RA, Kuiken T, Schutten M. Aetiology: Koch's postulates fulfilled for SARS virus. Nature. 2003;423:240. - PMC - PubMed

Publication types

MeSH terms

LinkOut - more resources