Notch and Ras signaling pathway effector genes expressed in fusion competent and founder cells during Drosophila myogenesis
- PMID: 14602676
- DOI: 10.1242/dev.00843
Notch and Ras signaling pathway effector genes expressed in fusion competent and founder cells during Drosophila myogenesis
Abstract
Drosophila muscles originate from the fusion of two types of myoblasts, founder cells (FCs) and fusion-competent myoblasts (FCMs). To better understand muscle diversity and morphogenesis, we performed a large-scale gene expression analysis to identify genes differentially expressed in FCs and FCMs. We employed embryos derived from Toll10b mutants to obtain primarily muscle-forming mesoderm, and expressed activated forms of Ras or Notch to induce FC or FCM fate, respectively. The transcripts present in embryos of each genotype were compared by hybridization to cDNA microarrays. Among the 83 genes differentially expressed, we found genes known to be enriched in FCs or FCMs, such as heartless or hibris, previously characterized genes with unknown roles in muscle development, and predicted genes of unknown function. Our studies of newly identified genes revealed new patterns of gene expression restricted to one of the two types of myoblasts, and also striking muscle phenotypes. Whereas genes such as phyllopod play a crucial role during specification of particular muscles, others such as tartan are necessary for normal muscle morphogenesis.
Similar articles
-
The immunoglobulin-like protein Hibris functions as a dose-dependent regulator of myoblast fusion and is differentially controlled by Ras and Notch signaling.Development. 2001 Nov;128(21):4251-64. doi: 10.1242/dev.128.21.4251. Development. 2001. PMID: 11684661
-
Multiple roles for notch in Drosophila myogenesis.Dev Biol. 1998 Sep 1;201(1):66-77. doi: 10.1006/dbio.1998.8944. Dev Biol. 1998. PMID: 9733574
-
Distinct genetic programs guide Drosophila circular and longitudinal visceral myoblast fusion.BMC Cell Biol. 2014 Jul 8;15:27. doi: 10.1186/1471-2121-15-27. BMC Cell Biol. 2014. PMID: 25000973 Free PMC article.
-
Determination and development of the larval muscle pattern in Drosophila melanogaster.Cell Tissue Res. 1999 Apr;296(1):151-60. doi: 10.1007/s004410051276. Cell Tissue Res. 1999. PMID: 10199975 Review.
-
Collective Migrations of Drosophila Embryonic Trunk and Caudal Mesoderm-Derived Muscle Precursor Cells.Genetics. 2020 Jun;215(2):297-322. doi: 10.1534/genetics.120.303258. Genetics. 2020. PMID: 32487692 Free PMC article. Review.
Cited by
-
From Drosophila to Human: Biological Function of E3 Ligase Godzilla and Its Role in Disease.Cells. 2022 Jan 23;11(3):380. doi: 10.3390/cells11030380. Cells. 2022. PMID: 35159190 Free PMC article. Review.
-
Complex interaction of Drosophila GRIP PDZ domains and Echinoid during muscle morphogenesis.EMBO J. 2006 Aug 9;25(15):3640-51. doi: 10.1038/sj.emboj.7601216. Epub 2006 Jul 20. EMBO J. 2006. PMID: 16858411 Free PMC article.
-
Discrete levels of Twist activity are required to direct distinct cell functions during gastrulation and somatic myogenesis.PLoS One. 2014 Jun 10;9(6):e99553. doi: 10.1371/journal.pone.0099553. eCollection 2014. PLoS One. 2014. PMID: 24915423 Free PMC article.
-
3D analysis of founder cell and fusion competent myoblast arrangements outlines a new model of myoblast fusion.Dev Biol. 2007 Sep 1;309(1):113-25. doi: 10.1016/j.ydbio.2007.06.024. Epub 2007 Jul 6. Dev Biol. 2007. PMID: 17662708 Free PMC article.
-
RNA polymerase II kinetics in polo polyadenylation signal selection.EMBO J. 2011 May 20;30(12):2431-44. doi: 10.1038/emboj.2011.156. EMBO J. 2011. PMID: 21602789 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
