doi: 10.1016/0014-5793(92)81468-2.
'All-or-none' mechanism of the molten globule unfolding
Affiliations
- PMID: 1451808
- DOI: 10.1016/0014-5793(92)81468-2
Item in Clipboard
'All-or-none' mechanism of the molten globule unfolding
FEBS Lett.
.
Free article
Abstract
The Gdm-HCl-induced unfolding of bovine carbonic anhydrase B and S. aureus beta-lactamase was studied at 4 degrees C by a variety of methods. With the use of FPLC it has been shown that within the transition from the molten globule to the unfolded state the distribution function of molecular dimensions is bimodal. This means that equilibrium intermediates between the molten globule and the unfolded states are absent, i.e. the molten globule unfolding follows the 'all-or-none' mechanism.
Similar articles
-
Further evidence on the equilibrium "pre-molten globule state": four-state guanidinium chloride-induced unfolding of carbonic anhydrase B at low temperature.J Mol Biol. 1996 Jan 12;255(1):215-28. doi: 10.1006/jmbi.1996.0018. J Mol Biol. 1996. PMID: 8568868
-
[Molten globule unfolding by strong denaturing agents occurs by the "all or nothing" principle].Biofizika. 1993 Jan-Feb;38(1):37-46. Biofizika. 1993. PMID: 8471644 Russian.
-
"Partly folded" state, a new equilibrium state of protein molecules: four-state guanidinium chloride-induced unfolding of beta-lactamase at low temperature.Biochemistry. 1994 Mar 15;33(10):2782-91. doi: 10.1021/bi00176a006. Biochemistry. 1994. PMID: 8130190
-
Structural energetics of the molten globule state.Proteins. 1993 Jun;16(2):115-40. doi: 10.1002/prot.340160202. Proteins. 1993. PMID: 8332604 Review.
-
Molten globule and protein folding.Adv Protein Chem. 1995;47:83-229. doi: 10.1016/s0065-3233(08)60546-x. Adv Protein Chem. 1995. PMID: 8561052 Review. No abstract available.
Cited by
-
Destabilizing an interacting motif strengthens the association of a designed ankyrin repeat protein with tubulin.Sci Rep. 2016 Jul 6;6:28922. doi: 10.1038/srep28922. Sci Rep. 2016. PMID: 27380724 Free PMC article.
-
Unfolding of CPR3 Gets Initiated at the Active Site and Proceeds via Two Intermediates.Biophys J. 2017 Feb 28;112(4):605-619. doi: 10.1016/j.bpj.2016.12.020. Biophys J. 2017. PMID: 28256221 Free PMC article.
-
A superior drug carrier--aponeocarzinostatin in partially unfolded state fully protects the labile antitumor enediyne.J Biomed Sci. 2009 May 23;16(1):48. doi: 10.1186/1423-0127-16-48. J Biomed Sci. 2009. PMID: 19463188 Free PMC article.
-
Heterogeneity of equilibrium molten globule state of cytochrome c induced by weak salt denaturants under physiological condition.PLoS One. 2015 Apr 7;10(4):e0120465. doi: 10.1371/journal.pone.0120465. eCollection 2015. PLoS One. 2015. PMID: 25849212 Free PMC article.
-
Activation of chicken liver dihydrofolate reductase by urea and guanidine hydrochloride is accompanied by conformational change at the active site.Biochem J. 1996 Apr 1;315 ( Pt 1)(Pt 1):97-102. doi: 10.1042/bj3150097. Biochem J. 1996. PMID: 8670138 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
