doi: 10.1046/j.1365-201X.2003.01188.x.
Lack of efficacy of 'naked' small interfering RNA applied directly to rat brain
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- PMID: 14510781
- DOI: 10.1046/j.1365-201X.2003.01188.x
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Lack of efficacy of 'naked' small interfering RNA applied directly to rat brain
Acta Physiol Scand.
2003 Oct.
Abstract
The intrastriatal infusions of 'naked' small interfering RNA (siRNA) targeted to dopamine D1 receptors (1.0-10.0 nmol over 3 days) did not reduce dopamine D1 receptor messenger RNA levels or receptor protein, assessed by [125I] SCH 23982 binding in intact rats. This was in contrast to results in vitro where a 76% reduction in dopamine D1 receptor ligand binding could be observed.
Conclusion: The results suggest that synthetic siRNA, when applied directly to rat brain, is not capable of inducing RNA interference.
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