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. 2003 Sep 19;114(6):701-13.
doi: 10.1016/s0092-8674(03)00714-1.

The protein network of HIV budding

Uta K von Schwedler  1 , Melissa Stuchell, Barbara Müller, Diane M Ward, Hyo-Young Chung, Eiji Morita, Hubert E Wang, Thaylon Davis, Gong-Ping He, Daniel M Cimbora, Anna Scott, Hans-Georg Kräusslich, Jerry Kaplan, Scott G Morham, Wesley I Sundquist
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Free article

The protein network of HIV budding

Uta K von Schwedler et al. Cell. .
Free article

Abstract

HIV release requires TSG101, a cellular factor that sorts proteins into vesicles that bud into multivesicular bodies (MVB). To test whether other proteins involved in MVB biogenesis (the class E proteins) also participate in HIV release, we identified 22 candidate human class E proteins. These proteins were connected into a coherent network by 43 different protein-protein interactions, with AIP1 playing a key role in linking complexes that act early (TSG101/ESCRT-I) and late (CHMP4/ESCRT-III) in the pathway. AIP1 also binds the HIV-1 p6(Gag) and EIAV p9(Gag) proteins, indicating that it can function directly in virus budding. Human class E proteins were found in HIV-1 particles, and dominant-negative mutants of late-acting human class E proteins arrested HIV-1 budding through plasmal and endosomal membranes. These studies define a protein network required for human MVB biogenesis and indicate that the entire network participates in the release of HIV and probably many other viruses.

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