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. 1992 Oct 2;71(1):97-105.
doi: 10.1016/0092-8674(92)90269-i.

The translation machinery and 70 kd heat shock protein cooperate in protein synthesis

R J Nelson  1 , T Ziegelhoffer, C Nicolet, M Werner-Washburne, E A Craig
Affiliations

The translation machinery and 70 kd heat shock protein cooperate in protein synthesis

R J Nelson et al. Cell. .

Abstract

The function of the yeast SSB 70 kd heatshock proteins (hsp70s) was investigated by a variety of approaches. The SSB hsp70s (Ssb1/2p) are associated with translating ribosomes. This association is disrupted by puromycin, suggesting that Ssb1/2p may bind directly to the nascent polypeptide. Mutant ssb1 ssb2 strains grow slowly, contain a low number of translating ribosomes, and are hypersensitive to several inhibitors of protein synthesis. The slow growth phenotype of ssb1 ssb2 mutants is suppressed by increased copy number of a gene encoding a novel translation elongation factor 1 alpha (EF-1 alpha)-like protein. We suggest that cytosolic hsp70 aids in the passage of the nascent polypeptide chain through the ribosome in a manner analogous to the role played by organelle-localized hsp70 in the transport of proteins across membranes.

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