Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Nov;66(11):6784-7.
doi: 10.1128/JVI.66.11.6784-6787.1992.

Construction and characterization of a human cytomegalovirus mutant with the UL18 (class I homolog) gene deleted

H Browne  1 , M Churcher, T Minson
Affiliations

Construction and characterization of a human cytomegalovirus mutant with the UL18 (class I homolog) gene deleted

H Browne et al. J Virol. 1992 Nov.

Abstract

The UL18 open reading frame of human cytomegalovirus (HCMV) (which encodes a product homologous to major histocompatibility complex class I heavy chains) has been disrupted by insertion of the beta-galactosidase gene under control of the major HCMV early promoter. The recombinant virus delta UL18 showed no phenotypic differences from wild-type HCMV in terms of single-step growth curves or particle/infectivity ratios, indicating that the UL18 gene product is dispensable for the growth of HCMV in human fibroblasts in vitro. The synthesis of the mature cellular class I heterodimer is shut down in cells infected at a high multiplicity with wild-type HCMV, and a similar effect was seen in delta UL18-infected fibroblasts, suggesting that although the UL18 gene product can associate with beta 2 microglobulin, it is not directly involved in the disruption of class I assembly.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Eur J Immunol. 1979 Jul;9(7):536-45 - PubMed
    1. Virology. 1963 Mar;19:250-60 - PubMed
    1. J Virol. 1992 Apr;66(4):2541-6 - PubMed
    1. J Virol. 1992 Nov;66(11):6609-15 - PubMed
    1. J Gen Virol. 1991 Nov;72 ( Pt 11):2757-64 - PubMed

Publication types