Studies on the intracellular mechanism of action of alpha-melanocyte-stimulating hormone on rat adrenal zona glomerulosa
- PMID: 1325151
- DOI: 10.1677/jme.0.0090047
Studies on the intracellular mechanism of action of alpha-melanocyte-stimulating hormone on rat adrenal zona glomerulosa
Abstract
The intracellular mechanisms of action of alpha-MSH in rat adrenocortical cells were examined. When rat adrenal capsule (largely glomerulosa) cells were stimulated with a range of concentrations of alpha-MSH there was significant stimulation of aldosterone secretion at 10(-10) mol/l, although cyclic AMP was not increased until high concentrations of alpha-MSH were used (10(-6) mol/l and above). However, cells incubated with ACTH showed an increase in aldosterone secretion at 10(-11) mol/l and levels of cyclic AMP were elevated at 10(-9) mol ACTH/l. When rat adrenal whole capsules were incubated with alpha-MSH, membrane-bound protein kinase C (PKC) activity was increased and cytosolic enzyme activity decreased, showing PKC activation. Stimulation with angiotensin II also induced translocation of PKC activity, but ACTH did not. When [3H]inositol-loaded glomerulosa cells were stimulated with alpha-MSH there was significant generation of [3H]inositol trisphosphate (IP3) at concentrations of alpha-MSH which stimulated secretion of aldosterone. Significantly increased levels of [3H]IP3 were also measured when loaded cells were exposed to angiotensin II. ACTH did not cause any significant stimulation of [3H]IP3 production at any concentration used. These results indicate that activation of PKC and phospholipase C is important in modulating the steroidogenic effect of alpha-MSH.
Similar articles
-
Role of tyrosine kinase and protein kinase C in the steroidogenic actions of angiotensin II, alpha-melanocyte-stimulating hormone and corticotropin in the rat adrenal cortex.Biochem J. 1995 Jan 15;305 ( Pt 2)(Pt 2):433-8. doi: 10.1042/bj3050433. Biochem J. 1995. PMID: 7832756 Free PMC article.
-
Alpha-melanocyte-stimulating hormone-induced inhibition of angiotensin II receptor-mediated events in the rat adrenal zona glomerulosa.J Mol Endocrinol. 1994 Aug;13(1):95-104. doi: 10.1677/jme.0.0130095. J Mol Endocrinol. 1994. PMID: 7999258
-
alpha-MSH and zona glomerulosa function in the rat.J Steroid Biochem. 1983 Jul;19(1B):537-44. doi: 10.1016/0022-4731(83)90215-7. J Steroid Biochem. 1983. PMID: 6310242
-
Recent progress in understanding aldosterone secretion.Gen Pharmacol. 1997 May;28(5):647-51. doi: 10.1016/s0306-3623(96)00290-x. Gen Pharmacol. 1997. PMID: 9184796 Review.
-
Reciprocal influences between the signalling pathways regulating proliferation and steroidogenesis in adrenal glomerulosa cells.J Mol Endocrinol. 2004 Jun;32(3):893-902. doi: 10.1677/jme.0.0320893. J Mol Endocrinol. 2004. PMID: 15171720 Review.
Cited by
-
Functional Zonation of the Adult Mammalian Adrenal Cortex.Front Neurosci. 2016 Jun 15;10:238. doi: 10.3389/fnins.2016.00238. eCollection 2016. Front Neurosci. 2016. PMID: 27378832 Free PMC article. Review.
-
Inhibition of endothelin- and phorbol ester-stimulated tyrosine kinase activity by corticotrophin in the rat adrenal zona glomerulosa.Biochem J. 1996 Feb 1;313 ( Pt 3)(Pt 3):867-72. doi: 10.1042/bj3130867. Biochem J. 1996. PMID: 8611168 Free PMC article.
-
Intracellular potentiation between two second messenger systems may contribute to cholera toxin induced intestinal secretion in humans.Gut. 2004 Jan;53(1):50-7. doi: 10.1136/gut.53.1.50. Gut. 2004. PMID: 14684576 Free PMC article.
-
Role of tyrosine kinase and protein kinase C in the steroidogenic actions of angiotensin II, alpha-melanocyte-stimulating hormone and corticotropin in the rat adrenal cortex.Biochem J. 1995 Jan 15;305 ( Pt 2)(Pt 2):433-8. doi: 10.1042/bj3050433. Biochem J. 1995. PMID: 7832756 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources