Nuclear transport of influenza virus polymerase PA protein
- PMID: 1320800
- DOI: 10.1016/0168-1702(92)90031-4
Nuclear transport of influenza virus polymerase PA protein
Abstract
The subcellular distribution of influenza polymerase PA subunit has been studied using a SV40-recombinant virus (SVPA76), which allows the expression and accumulation of this protein in COS-1 cells. In contrast to the complete nuclear localization observed for the PA subunit several hours after influenza virus infection, when COS-1 cells were infected with the SVPA76 recombinant, the PA protein accumulated either in the nucleus, in the cytoplasm or was distributed throughout the cell. When cells were infected with the SVPA76 recombinant and superinfected with influenza virus, a clear increase in the proportion of cells showing nuclear localization of the PA protein was observed, suggesting that some trans-factor may be required to allow complete nuclear accumulation of the protein. Double infections using SVPA76 recombinant and either SVPB1 or SVNS recombinant viruses showed a complete correlation between expression of polymerase PB1 subunit or NS1 protein and nuclear localization of polymerase PA subunit. However, no such correlation was observed in the double infections of SVPA76 and SVNP recombinants. These results suggest that polymerase PB1 subunit and the non-structural proteins could be involved in the nuclear targeting or nuclear retention of influenza polymerase PA protein.
Similar articles
-
Nucleocytoplasmic transport: the influenza virus NS1 protein regulates the transport of spliced NS2 mRNA and its precursor NS1 mRNA.Genes Dev. 1992 Feb;6(2):255-67. doi: 10.1101/gad.6.2.255. Genes Dev. 1992. PMID: 1531330
-
Complex structure of the nuclear translocation signal of influenza virus polymerase PA subunit.J Gen Virol. 1994 Jan;75 ( Pt 1):29-36. doi: 10.1099/0022-1317-75-1-29. J Gen Virol. 1994. PMID: 8113737
-
Intracellular localization of the viral polymerase proteins in cells infected with influenza virus and cells expressing PB1 protein from cloned cDNA.J Virol. 1987 Jul;61(7):2217-24. doi: 10.1128/JVI.61.7.2217-2224.1987. J Virol. 1987. PMID: 2438429 Free PMC article.
-
Nuclear traffic of influenza virus proteins and ribonucleoprotein complexes.Virus Res. 2007 Mar;124(1-2):12-21. doi: 10.1016/j.virusres.2006.09.013. Epub 2006 Nov 1. Virus Res. 2007. PMID: 17081640 Review.
-
The molecular anatomy of influenza virus RNA polymerase.Biol Chem. 1997 Jun;378(6):483-8. Biol Chem. 1997. PMID: 9224927 Review.
Cited by
-
A complicated message: Identification of a novel PB1-related protein translated from influenza A virus segment 2 mRNA.J Virol. 2009 Aug;83(16):8021-31. doi: 10.1128/JVI.00826-09. Epub 2009 Jun 3. J Virol. 2009. PMID: 19494001 Free PMC article.
-
The human H5N1 influenza A virus polymerase complex is active in vitro over a broad range of temperatures, in contrast to the WSN complex, and this property can be attributed to the PB2 subunit.J Gen Virol. 2008 Dec;89(Pt 12):2923-2932. doi: 10.1099/vir.0.2008/006254-0. J Gen Virol. 2008. PMID: 19008377 Free PMC article.
-
Two years after pandemic influenza A/2009/H1N1: what have we learned?Clin Microbiol Rev. 2012 Apr;25(2):223-63. doi: 10.1128/CMR.05012-11. Clin Microbiol Rev. 2012. PMID: 22491771 Free PMC article. Review.
-
Involvement of Hsp90 in assembly and nuclear import of influenza virus RNA polymerase subunits.J Virol. 2007 Feb;81(3):1339-49. doi: 10.1128/JVI.01917-06. Epub 2006 Nov 22. J Virol. 2007. PMID: 17121807 Free PMC article.
-
Cellular protein HAX1 interacts with the influenza A virus PA polymerase subunit and impedes its nuclear translocation.J Virol. 2013 Jan;87(1):110-23. doi: 10.1128/JVI.00939-12. Epub 2012 Oct 10. J Virol. 2013. PMID: 23055567 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
