doi: 10.1016/0166-0934(92)90146-5.
Glycerol shock treatment facilitates purification of herpes simplex virus
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- PMID: 1313439
- DOI: 10.1016/0166-0934(92)90146-5
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Glycerol shock treatment facilitates purification of herpes simplex virus
J Virol Methods.
1992 Feb.
Abstract
We describe an improved method for purification of herpes simplex virus. The primary improvement is the use of glycerol shock treatment to lyse infected cells. This is a rapid and simple technique that efficiently extracts the cytoplasm from the nucleus, while preserving nuclear integrity. Nearly 100% cell lysis is achieved by this method, and cytoplasmic virus yield is optimal. Other improvements to increase virus yield and purity and to economize on equipment are described.
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