Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Mar;116(6):1343-55.
doi: 10.1083/jcb.116.6.1343.

Evidence for the regulation of exocytic transport by protein phosphorylation

H W Davidson  1 , C H McGowan, W E Balch
Affiliations

Evidence for the regulation of exocytic transport by protein phosphorylation

H W Davidson et al. J Cell Biol. 1992 Mar.

Abstract

We investigated the effects of the protein phosphatase inhibitors okadaic acid and microcystin-LR upon transport of newly synthesized proteins through the exocytic pathway. Treatment of CHO cells with 1 microM okadaic acid rapidly inhibited movement of a marker protein (vesicular stomatitis virus G protein) from the endoplasmic reticulum to the Golgi compartment. Both okadaic acid and microcystin-LR also inhibited transport in an in vitro assay reconstituting movement to the Golgi compartment, at concentrations equivalent to those required to inhibit phosphorylase phosphatase activity. Inhibition both in vivo and in vitro could be antagonized by protein kinase inhibitors, suggesting that protein phosphorylation was directly responsible for this effect. An early stage in the transport reaction associated with vesicle formation or targeting was inhibited by protein phosphorylation, which could be reversed by fractions enriched in protein phosphatase 2A. Protein kinase antagonists did not inhibit transport between sequential compartments of the exocytic pathway in vitro, suggesting that protein phosphorylation is not itself required for vesicular transport. During mitosis, vesicular transport is inhibited simultaneous to the activation of maturation-promoting factor. It is proposed that the inhibition caused by okadaic acid and microcystin-LR involves a similar mechanism to that responsible for the mitotic arrest of vesicular transport.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Nature. 1988 Oct 13;335(6191):592-3 - PubMed
    1. Eur J Biochem. 1987 Aug 17;167(1):135-40 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Dec;84(23):8210-4 - PubMed
    1. Biochem Biophys Res Commun. 1986 Mar 13;135(2):397-402 - PubMed
    1. J Gen Virol. 1970 Sep;8(3):187-95 - PubMed

Publication types

MeSH terms