Schizosaccharomyces pombe cells deficient in triacylglycerols synthesis undergo apoptosis upon entry into the stationary phase
- PMID: 12963726
- DOI: 10.1074/jbc.M306998200
Schizosaccharomyces pombe cells deficient in triacylglycerols synthesis undergo apoptosis upon entry into the stationary phase
Abstract
Triacylglycerols (TAG) are important energy storage molecules for nearly all eukaryotic organisms. In this study, we found that two gene products (Plh1p and Dga1p) are responsible for the terminal step of TAG synthesis in the fission yeast Schizosaccharomyces pombe through two different mechanisms: Plh1p is a phospholipid diacylglycerol acyltransferase, whereas Dga1p is an acyl-CoA:diacylglycerol acyltransferase. Cells with both dga1+ and plh1+ deleted (DKO cells) lost viability upon entry into the stationary phase and demonstrated prominent apoptotic markers. Exponentially growing DKO cells also underwent dramatic apoptosis when briefly treated with diacylglycerols (DAGs) or free fatty acids. We provide strong evidence suggesting that DAG, not sphingolipids, mediates fatty acids-induced lipoapoptosis in yeast. Lastly, we show that generation of reactive oxygen species is essential to lipoapoptosis.
Similar articles
-
Synthesis of triacylglycerols by the acyl-coenzyme A:diacyl-glycerol acyltransferase Dga1p in lipid particles of the yeast Saccharomyces cerevisiae.J Bacteriol. 2002 Jan;184(2):519-24. doi: 10.1128/JB.184.2.519-524.2002. J Bacteriol. 2002. PMID: 11751830 Free PMC article.
-
Expression in yeast and tobacco of plant cDNAs encoding acyl CoA:diacylglycerol acyltransferase.Eur J Biochem. 2000 Jan;267(1):85-96. doi: 10.1046/j.1432-1327.2000.00961.x. Eur J Biochem. 2000. PMID: 10601854
-
Triacylglycerol biosynthesis in yeast.Appl Microbiol Biotechnol. 2003 May;61(4):289-99. doi: 10.1007/s00253-002-1212-4. Epub 2003 Jan 29. Appl Microbiol Biotechnol. 2003. PMID: 12743757 Review.
-
Apoptosis and lipoapoptosis in the fission yeast Schizosaccharomyces pombe.FEMS Yeast Res. 2005 Dec;5(12):1199-206. doi: 10.1016/j.femsyr.2005.07.004. Epub 2005 Aug 24. FEMS Yeast Res. 2005. PMID: 16137929 Review.
-
Lipid Droplets Form from Distinct Regions of the Cell in the Fission Yeast Schizosaccharomyces pombe.Traffic. 2016 Jun;17(6):657-69. doi: 10.1111/tra.12394. Epub 2016 Apr 29. Traffic. 2016. PMID: 26990381 Free PMC article.
Cited by
-
Oleate inhibits steryl ester synthesis and causes liposensitivity in yeast.J Biol Chem. 2010 Aug 27;285(35):26832-26841. doi: 10.1074/jbc.M110.122085. Epub 2010 Jun 22. J Biol Chem. 2010. PMID: 20571028 Free PMC article.
-
Seipin concentrates distinct neutral lipids via interactions with their acyl chain carboxyl esters.J Cell Biol. 2022 Sep 5;221(9):e202112068. doi: 10.1083/jcb.202112068. Epub 2022 Aug 8. J Cell Biol. 2022. PMID: 35938957 Free PMC article.
-
Lipids Affect the Cryptococcus neoformans-Macrophage Interaction and Promote Nonlytic Exocytosis.Infect Immun. 2017 Nov 17;85(12):e00564-17. doi: 10.1128/IAI.00564-17. Print 2017 Dec. Infect Immun. 2017. PMID: 28947642 Free PMC article.
-
The BAR domain proteins: molding membranes in fission, fusion, and phagy.Microbiol Mol Biol Rev. 2006 Mar;70(1):37-120. doi: 10.1128/MMBR.70.1.37-120.2006. Microbiol Mol Biol Rev. 2006. PMID: 16524918 Free PMC article. Review.
-
Good fat, essential cellular requirements for triacylglycerol synthesis to maintain membrane homeostasis in yeast.J Biol Chem. 2009 Nov 6;284(45):30981-93. doi: 10.1074/jbc.M109.024752. Epub 2009 Jul 16. J Biol Chem. 2009. PMID: 19608739 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
