Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2003 Sep;84(Pt 9):2511-2516.
doi: 10.1099/vir.0.19298-0.

The most abundantly transcribed human cytomegalovirus gene (beta 2.7) is non-essential for growth in vitro

Brian P McSharry  1 , Peter Tomasec  1 , M Lynne Neale  2 , Gavin W G Wilkinson  1
Affiliations
Comparative Study

The most abundantly transcribed human cytomegalovirus gene (beta 2.7) is non-essential for growth in vitro

Brian P McSharry et al. J Gen Virol. 2003 Sep.

Abstract

The most abundantly transcribed HCMV gene (beta 2.7) encodes a 2.7 kb polyadenylated RNA. Although the laboratory-adapted HCMV strains AD169 and Towne possess two copies of the beta 2.7 gene within an expanded b sequence element, the low passage strain Toledo and all clinical isolates analysed contain only a single copy located within the U(L) region. A beta 2.7 deletion mutant constructed based on a strain Toledo background was shown to replicate with kinetics comparable to those of the parental virus; the beta2.7 gene is therefore not essential for virus replication in vitro. Sequencing the beta 2.7 gene from HCMV clinical isolates and the Toledo strain reveals that although the overall gene sequence is highly conserved (>99 %), the RL4 frame originally assigned in strain AD169 was disrupted in each of these viruses. Consequently, the beta 2.7 transcript does not encode any obvious translation product and thus may not function as an mRNA.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

Publication types

Associated data

LinkOut - more resources