Fundamental role of ClC-3 in volume-sensitive Cl- channel function and cell volume regulation in AGS cells
- PMID: 12842831
- DOI: 10.1152/ajpgi.00470.2002
Fundamental role of ClC-3 in volume-sensitive Cl- channel function and cell volume regulation in AGS cells
Abstract
Volume regulation is essential for cell function, but it is unknown which channels are involved in a regulatory volume decrease (RVD) in human gastric epithelial cells. Exposure to a hypotonic solution caused the increase in AGS cell volume, followed by the activation of a current. The reversal potential of the swelling-induced current suggested that Cl- was the primary charge carrier. The selectivity sequence for different anions was I- > Br- > Cl- > F- > gluconate. This current was inhibited by flufenamate, DIDS, tamoxifen, and 5-nitro-2-(3-phenylpropylamino)benzoate. Intracellular dialysis of three different anti-ClC-3 antibodies abolished or attenuated the Cl- current and disrupted RVD, whereas the current and RVD was unaltered by anti-ClC-2 antibody. Immunoblot studies demonstrated the presence of ClC-3 protein in Hela and AGS cells. RT-PCR analysis detected expression of ClC-3, MDR-1, and pICln mRNA in AGS cells. These results suggest a fundamental role of endogenous ClC-3 in the swelling-activated Cl- channels function and cell volume regulation in human gastric epithelial cells.
Similar articles
-
Role of volume-stimulated osmolyte and anion channels in volume regulation by mammalian sperm.Mol Hum Reprod. 2004 Nov;10(11):815-23. doi: 10.1093/molehr/gah106. Epub 2004 Sep 10. Mol Hum Reprod. 2004. PMID: 15361553
-
A role for the volume regulated anion channel in volume regulation in the murine CNS cell line, CAD.Acta Physiol (Oxf). 2010 Feb;198(2):159-68. doi: 10.1111/j.1748-1716.2009.02050.x. Epub 2009 Oct 7. Acta Physiol (Oxf). 2010. PMID: 19811460
-
Volume regulation in the bovine lens and cataract. The involvement of chloride channels.J Clin Invest. 1996 Feb 15;97(4):971-8. doi: 10.1172/JCI118521. J Clin Invest. 1996. PMID: 8613551 Free PMC article.
-
Expression and function of CLC and cystic fibrosis transmembrane conductance regulator chloride channels in renal epithelial tubule cells: pathophysiological implications.Chang Gung Med J. 2007 Jan-Feb;30(1):17-25. Chang Gung Med J. 2007. PMID: 17477025 Review.
-
ICln, an ion channel-forming protein associated with cell volume regulation.Exp Physiol. 1999 Nov;84(6):1023-31. Exp Physiol. 1999. PMID: 10564699 Review.
Cited by
-
Cell cycle-dependent subcellular distribution of ClC-3 in HeLa cells.Histochem Cell Biol. 2012 Jun;137(6):763-76. doi: 10.1007/s00418-012-0937-0. Epub 2012 Feb 28. Histochem Cell Biol. 2012. PMID: 22371056
-
Altered properties of volume-sensitive osmolyte and anion channels (VSOACs) and membrane protein expression in cardiac and smooth muscle myocytes from Clcn3-/- mice.J Physiol. 2004 Jun 1;557(Pt 2):439-56. doi: 10.1113/jphysiol.2003.059261. Epub 2004 Mar 12. J Physiol. 2004. PMID: 15020697 Free PMC article.
-
Transient receptor potential melastatin 4 inhibitor 9-phenanthrol abolishes arrhythmias induced by hypoxia and re-oxygenation in mouse ventricle.Br J Pharmacol. 2012 Apr;165(7):2354-64. doi: 10.1111/j.1476-5381.2011.01715.x. Br J Pharmacol. 2012. PMID: 22014185 Free PMC article.
-
Overexpression of chloride channel-3 predicts unfavorable prognosis and promotes cellular invasion in gastric cancer.Cancer Manag Res. 2018 May 14;10:1163-1175. doi: 10.2147/CMAR.S159790. eCollection 2018. Cancer Manag Res. 2018. Retraction in: Cancer Manag Res. 2024 May 16;16:465-466. doi: 10.2147/CMAR.S478202 PMID: 29795988 Free PMC article. Retracted.
-
Flufenamic acid as an ion channel modulator.Pharmacol Ther. 2013 May;138(2):272-84. doi: 10.1016/j.pharmthera.2013.01.012. Epub 2013 Jan 25. Pharmacol Ther. 2013. PMID: 23356979 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
