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. 2003 May;4(5):753-9.
doi: 10.1016/s1534-5807(03)00122-9.

Syntaxin 2 and endobrevin are required for the terminal step of cytokinesis in mammalian cells

Seng Hui Low  1 , Xin Li, Masumi Miura, Noriko Kudo, Beatriz Quiñones, Thomas Weimbs
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Free article

Syntaxin 2 and endobrevin are required for the terminal step of cytokinesis in mammalian cells

Seng Hui Low et al. Dev Cell. 2003 May.
Free article

Abstract

The terminal step of cytokinesis in animal cells is the abscission of the midbody, a cytoplasmic bridge that connects the two prospective daughter cells. Here we show that two members of the SNARE membrane fusion machinery, syntaxin 2 and endobrevin/VAMP-8, specifically localize to the midbody during cytokinesis in mammalian cells. Inhibition of their function by overexpression of nonmembrane-anchored mutants causes failure of cytokinesis leading to the formation of binucleated cells. Time-lapse microscopy shows that only midbody abscission but not further upstream events, such as furrowing, are affected. These results indicate that successful completion of cytokinesis requires a SNARE-mediated membrane fusion event and that this requirement is distinct from exocytic events that may be involved in prior ingression of the plasma membrane.

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