Analysis of clonotype distribution and persistence for an influenza virus-specific CD8+ T cell response
- PMID: 12705857
- DOI: 10.1016/s1074-7613(03)00087-6
Analysis of clonotype distribution and persistence for an influenza virus-specific CD8+ T cell response
Abstract
The spectrum of TCR V beta usage is compared for primary and recall CD8(+)D(b)PA(224)(+) T cell responses in mice with influenza pneumonia. Single-cell RT-PCR established that the same clonotypes were present in the lymphoid tissue and in the virus-infected lung. Longitudinal analysis indicated that the memory TCR repertoire reflects the primary response, with no decrease in diversity prior to (or after) secondary challenge. The re-engagement of memory T cells looked to be stochastic in this localized, transient infection. Analysis of clonotypes from the blood, spleen, regional lymph nodes, bone marrow, lung, and liver over a 200 day interval showed no evidence of selective localization or loss. The long-term distribution of memory T cells seemed to be essentially random.
Similar articles
-
Prominent usage of V beta 8.3 T cells in the H-2Db-restricted response to an influenza A virus nucleoprotein epitope.J Immunol. 1993 Sep 1;151(5):2658-66. J Immunol. 1993. PMID: 7689611
-
Quantitative analysis of the influenza virus-specific CD4+ T cell memory in the absence of B cells and Ig.J Immunol. 1996 Oct 1;157(7):2947-52. J Immunol. 1996. PMID: 8816401
-
Fixing an irrelevant TCR alpha chain reveals the importance of TCR beta diversity for optimal TCR alpha beta pairing and function of virus-specific CD8+ T cells.Eur J Immunol. 2010 Sep;40(9):2470-81. doi: 10.1002/eji.201040473. Eur J Immunol. 2010. PMID: 20690181
-
Characterization of CD8+ T cell repertoire diversity and persistence in the influenza A virus model of localized, transient infection.Semin Immunol. 2004 Jun;16(3):179-84. doi: 10.1016/j.smim.2004.02.005. Semin Immunol. 2004. PMID: 15130502 Review.
-
Establishment and recall of CD8+ T-cell memory in a model of localized transient infection.Immunol Rev. 2006 Jun;211:133-45. doi: 10.1111/j.0105-2896.2006.00386.x. Immunol Rev. 2006. PMID: 16824123 Review.
Cited by
-
Dynamics of the cytotoxic T cell response to a model of acute viral infection.J Virol. 2015 Apr;89(8):4517-26. doi: 10.1128/JVI.03474-14. Epub 2015 Feb 4. J Virol. 2015. PMID: 25653453 Free PMC article.
-
Identification of T cell clones without the need for sequencing.J Immunol Methods. 2015 Sep;424:28-31. doi: 10.1016/j.jim.2015.04.018. Epub 2015 May 8. J Immunol Methods. 2015. PMID: 25960175 Free PMC article.
-
Conserved T cell receptor usage in primary and recall responses to an immunodominant influenza virus nucleoprotein epitope.Proc Natl Acad Sci U S A. 2004 Apr 6;101(14):4942-7. doi: 10.1073/pnas.0401279101. Epub 2004 Mar 22. Proc Natl Acad Sci U S A. 2004. PMID: 15037737 Free PMC article.
-
Tracking phenotypically and functionally distinct T cell subsets via T cell repertoire diversity.Mol Immunol. 2008 Feb;45(3):607-18. doi: 10.1016/j.molimm.2006.05.017. Epub 2007 Aug 24. Mol Immunol. 2008. PMID: 17719639 Free PMC article. Review.
-
Location rather than CD62L phenotype is critical in the early establishment of influenza-specific CD8+ T cell memory.Proc Natl Acad Sci U S A. 2007 Jun 5;104(23):9782-7. doi: 10.1073/pnas.0703699104. Epub 2007 May 23. Proc Natl Acad Sci U S A. 2007. PMID: 17522251 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
