PARP-3 localizes preferentially to the daughter centriole and interferes with the G1/S cell cycle progression
- PMID: 12640039
- DOI: 10.1242/jcs.00341
PARP-3 localizes preferentially to the daughter centriole and interferes with the G1/S cell cycle progression
Abstract
A novel member of the poly(ADP-ribose) polymerase (PARP) family, hPARP-3, is identified here as a core component of the centrosome. hPARP-3 is preferentially localized to the daughter centriole throughout the cell cycle. The N-terminal domain (54 amino acids) of hPARP-3 is responsible for its centrosomal localization. Full-length hPAPR-3 (540 amino acids, with an apparent mass of 67 kDa) synthesizes ADP-ribose polymers during its automodification. Overexpression of hPARP-3 or its N-terminal domain does not influence centrosomal duplication or amplification but interferes with the G1/S cell cycle progression. PARP-1 also resides for part of the cell cycle in the centrosome and interacts with hPARP-3. The presence of both PARP-1 and PARP-3 at the centrosome may link the DNA damage surveillance network to the mitotic fidelity checkpoint.
Similar articles
-
DNA-induced dimerization of poly(ADP-ribose) polymerase-1 triggers its activation.Biochemistry. 2005 Nov 8;44(44):14670-81. doi: 10.1021/bi050755o. Biochemistry. 2005. PMID: 16262266
-
The expression of poly(ADP-ribose) polymerase during differentiation-linked DNA replication reveals that it is a component of the multiprotein DNA replication complex.Biochemistry. 1996 Sep 10;35(36):11622-33. doi: 10.1021/bi953010z. Biochemistry. 1996. PMID: 8794742
-
Poly(ADP-ribose) polymerase-1 dimerizes at a 5' recessed DNA end in vitro: a fluorescence study.Biochemistry. 2003 Oct 28;42(42):12409-17. doi: 10.1021/bi0344432. Biochemistry. 2003. PMID: 14567702
-
Centrosomes in the DNA damage response--the hub outside the centre.Chromosome Res. 2016 Jan;24(1):35-51. doi: 10.1007/s10577-015-9503-7. Chromosome Res. 2016. PMID: 26614090 Review.
-
The PLK4-STIL-SAS-6 module at the core of centriole duplication.Biochem Soc Trans. 2016 Oct 15;44(5):1253-1263. doi: 10.1042/BST20160116. Biochem Soc Trans. 2016. PMID: 27911707 Free PMC article. Review.
Cited by
-
Regulation of the poly(ADP-ribose) polymerase-1 gene expression by the transcription factors Sp1 and Sp3 is under the influence of cell density in primary cultured cells.Biochem J. 2005 Jul 15;389(Pt 2):423-33. doi: 10.1042/BJ20041718. Biochem J. 2005. PMID: 15777284 Free PMC article.
-
PARP3 affects the relative contribution of homologous recombination and nonhomologous end-joining pathways.Nucleic Acids Res. 2014 May;42(9):5616-32. doi: 10.1093/nar/gku174. Epub 2014 Mar 5. Nucleic Acids Res. 2014. PMID: 24598253 Free PMC article.
-
The underlying mechanism for the PARP and BRCA synthetic lethality: clearing up the misunderstandings.Mol Oncol. 2011 Aug;5(4):387-93. doi: 10.1016/j.molonc.2011.07.001. Epub 2011 Jul 22. Mol Oncol. 2011. PMID: 21821475 Free PMC article. Review.
-
A systematic analysis of the PARP protein family identifies new functions critical for cell physiology.Nat Commun. 2013;4:2240. doi: 10.1038/ncomms3240. Nat Commun. 2013. PMID: 23917125 Free PMC article.
-
FancJ regulates interstrand crosslinker induced centrosome amplification through the activation of polo-like kinase 1.Biol Open. 2013 Aug 6;2(10):1022-31. doi: 10.1242/bio.20135801. eCollection 2013. Biol Open. 2013. PMID: 24167712 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous
