Crystal structure of PU.1/IRF-4/DNA ternary complex
- PMID: 12453417
- DOI: 10.1016/s1097-2765(02)00703-7
Crystal structure of PU.1/IRF-4/DNA ternary complex
Abstract
The Ets and IRF transcription factor families contain structurally divergent members, PU.1, Spi-B and IRF-4 (Pip), IRF-8 (ICSBP), respectively, which have evolved to cooperatively assemble on composite DNA elements and regulate gene expression in the immune system. Whereas PU.1 recruits IRF-4 or IRF-8 to DNA, it exhibits an anticooperative interaction with IRF-1 and IRF-2. We report here the structure of the ternary complex formed with the DNA binding domains of PU.1 and IRF-4 on a composite DNA element. The DNA in the complex contorts into an unusual S shape that juxtaposes PU.1 and IRF-4 for selective electrostatic and hydrophobic interactions across the central minor groove. Together, the protein-protein and protein-DNA interactions provide insights into the stereochemical basis of cooperativity and anti-cooperativity between Ets and IRF factors.
Similar articles
-
Crystallization and characterization of PU.1/IRF-4/DNA ternary complex.J Struct Biol. 2002 Jul;139(1):55-9. doi: 10.1016/s1047-8477(02)00514-2. J Struct Biol. 2002. PMID: 12372320
-
Pip, a lymphoid-restricted IRF, contains a regulatory domain that is important for autoinhibition and ternary complex formation with the Ets factor PU.1.Genes Dev. 1996 Sep 15;10(18):2335-47. doi: 10.1101/gad.10.18.2335. Genes Dev. 1996. PMID: 8824592
-
Analysis of PU.1/ICSBP (IRF-8) complex formation with various PU.1 mutants: molecular cloning of rat Icsbp (Irf-8) cDNA.Immunogenetics. 2005 Mar;56(12):871-7. doi: 10.1007/s00251-004-0754-2. Epub 2005 Feb 2. Immunogenetics. 2005. PMID: 15688197
-
PU.1/Interferon Regulatory Factor interactions: mechanisms of transcriptional regulation.Cell Biochem Biophys. 2000;33(2):127-48. doi: 10.1385/CBB:33:2:127. Cell Biochem Biophys. 2000. PMID: 11325034 Review.
-
Immune cell-specific amplification of interferon signaling by the IRF-4/8-PU.1 complex.J Interferon Cytokine Res. 2005 Dec;25(12):770-9. doi: 10.1089/jir.2005.25.770. J Interferon Cytokine Res. 2005. PMID: 16375605 Review.
Cited by
-
Crystal structure of ATF-2/c-Jun and IRF-3 bound to the interferon-beta enhancer.EMBO J. 2004 Nov 10;23(22):4384-93. doi: 10.1038/sj.emboj.7600453. Epub 2004 Oct 28. EMBO J. 2004. PMID: 15510218 Free PMC article.
-
IRF-4,8 orchestrate the pre-B-to-B transition in lymphocyte development.Genes Dev. 2003 Jul 15;17(14):1703-8. doi: 10.1101/gad.1104803. Epub 2003 Jun 27. Genes Dev. 2003. PMID: 12832394 Free PMC article.
-
Missense mutations that cause Van der Woude syndrome and popliteal pterygium syndrome affect the DNA-binding and transcriptional activation functions of IRF6.Hum Mol Genet. 2009 Feb 1;18(3):535-45. doi: 10.1093/hmg/ddn381. Epub 2008 Nov 26. Hum Mol Genet. 2009. PMID: 19036739 Free PMC article.
-
Dendritic cell fate is determined by BCL11A.Proc Natl Acad Sci U S A. 2014 Mar 18;111(11):E998-1006. doi: 10.1073/pnas.1319228111. Epub 2014 Mar 3. Proc Natl Acad Sci U S A. 2014. PMID: 24591644 Free PMC article.
-
Inhibition of IL-1beta transcription by peptides derived from the hCMV IE2 transactivator.Mol Immunol. 2008 May;45(9):2667-77. doi: 10.1016/j.molimm.2007.12.024. Epub 2008 Mar 4. Mol Immunol. 2008. PMID: 18308397 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
