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Review
. 2002 Dec;120(6):773-9.
doi: 10.1085/jgp.20028704.

The gp91phox component of NADPH oxidase is not a voltage-gated proton channel

Thomas E DeCoursey  1 , Deri Morgan, Vladimir V Cherny
Affiliations
Review

The gp91phox component of NADPH oxidase is not a voltage-gated proton channel

Thomas E DeCoursey et al. J Gen Physiol. 2002 Dec.
No abstract available

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Figures

F<sc>igure</sc> 1.
Figure 1.
Cartoon illustrating the electron and proton currents during the respiratory burst in phagocytes. The two membrane-bound components, gp91phox and p22phox, occur together in phagocytes in a complex called flavocytochrome b 558 that includes FAD and two heme groups, and comprises an electron pathway. The conversion of H2O2 to HOCl is catalyzed by myeloperoxidase (MPO), which causes pus to be green. We have added an extra proton to both sides of the reaction that usually is written: NADPH → NADP+ + H+. This was done because (a) the regeneration of NADPH produces one additional intracellular proton, (b) the dismutation of O2 − to H2O2 consumes two extracellular protons, and (c) balancing the two translocated electrons requires two protons. This figure was modified from a version originally published in DeCoursey and Grinstein (1999).
F<sc>igure</sc> 2.
Figure 2.
Families of H+ currents recorded in neutrophils from a normal individual (top) and from a patient with CGD who lacked gp91phox expression (bottom). Voltage pulses were applied in 20-mV increments as labeled. Cells were studied in permeabilized patch configuration, with a symmetrical NH4 + gradient applied to clamp pHi to pHo, which was 7.0, as described (DeCoursey et al., 2001b). The currents on the left were recorded before stimulation, those on the right after stimulation with PMA. The average H+ current amplitudes are the same in CGD and normal cells (DeCoursey et al., 2001b). The constant small inward current at the holding potential in the normal cell after PMA treatment is due to electron current through the activated NADPH oxidase complex, and is absent in the CGD cell. The proton conductance in both resting and activated cells is the same in the presence or absence of gp91phox, which is inconsistent with the proposal that gp91phox is a proton channel. Figure from DeCoursey (2003).
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