doi: 10.1016/s1097-2765(02)00651-2.
Evidence that siRNAs function as guides, not primers, in the Drosophila and human RNAi pathways
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- PMID: 12408822
- DOI: 10.1016/s1097-2765(02)00651-2
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Evidence that siRNAs function as guides, not primers, in the Drosophila and human RNAi pathways
Mol Cell.
2002 Sep.
Free article
Abstract
In Drosophila, two features of small interfering RNA (siRNA) structure--5' phosphates and 3' hydroxyls--are reported to be essential for RNA interference (RNAi). Here, we show that as in Drosophila, a 5' phosphate is required for siRNA function in human HeLa cells. In contrast, we find no evidence in flies or humans for a role in RNAi for the siRNA 3' hydroxyl group. Our in vitro data suggest that in both flies and mammals, each siRNA guides endonucleolytic cleavage of the target RNA at a single site. We conclude that the underlying mechanism of RNAi is conserved between flies and mammals and that RNA-dependent RNA polymerases are not required for RNAi in these organisms.
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