Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2002 Sep;162(1):165-76.
doi: 10.1093/genetics/162.1.165.

Using the P[wHy] hybrid transposable element to disrupt genes in region 54D-55B in Drosophila melanogaster

Stephanie E Mohr  1 , William M Gelbart
Affiliations

Using the P[wHy] hybrid transposable element to disrupt genes in region 54D-55B in Drosophila melanogaster

Stephanie E Mohr et al. Genetics. 2002 Sep.

Abstract

Understanding the function of each gene in the genome of a model organism such as Drosophila melanogaster is an important goal. The development of improved methods for uncovering the mutant phenotypes of specific genes can accelerate achievement of this goal. The P[wHy] hybrid transposable element can be used to generate nested sets of precisely mapped deletions in a given region of the Drosophila genome. Here we use the P[wHy] method to generate overlapping, molecularly defined deletions from a set of three P[wHy] insertions in the 54E-F region of chromosome 2. Deletions that span a total of 0.5 Mb were identified and molecularly mapped precisely. Using overlapping deletions, the mutant phenotypes of nine previously uncharacterized genes in a 101-kb region were determined, including identification of new loci required for viability and female fertility. In addition, the deletions were used to molecularly map previously isolated lethal mutations. Thus, the P[wHy] method provides an efficient method for systematically determining the phenotypes of genes in a given region of the fly genome.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Science. 2000 Mar 24;287(5461):2185-95 - PubMed
    1. Cell. 1998 Dec 23;95(7):1017-26 - PubMed
    1. Science. 2000 Jun 16;288(5473):2013-8 - PubMed
    1. Genetics. 2000 Jul;155(3):1231-44 - PubMed
    1. J Cell Biol. 2000 Jul 24;150(2):F51-6 - PubMed

Publication types

LinkOut - more resources