Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1996 Feb;8(2):179-188.
doi: 10.1105/tpc.8.2.179.

Suppression of Virus Accumulation in Transgenic Plants Exhibiting Silencing of Nuclear Genes

J. J. English  1 , E. Mueller, D. C. Baulcombe
Affiliations

Suppression of Virus Accumulation in Transgenic Plants Exhibiting Silencing of Nuclear Genes

J. J. English et al. Plant Cell. 1996 Feb.

Abstract

Homology-dependent gene silencing contributes to variation between transgenic plants with respect to transgene and/or endogenous gene expression levels. Recent studies have linked post-transcriptional gene silencing and virus resistance in plants expressing virus-derived transgenes. Using a potato virus X vector, we present three examples in which silencing of nonviral transgenes prevented virus accumulation. This effect was dependent on sequence homology between the virus and the silenced transgene. Analysis of potato virus X derivatives carrying bacterial [beta]-glucuronidase (GUS) sequences showed that the 3[prime] region of the GUS coding sequence was a target of the silencing mechanism in two independent tobacco lines. Methylation of the silenced GUS transgenes in these lines was also concentrated in the 3[prime] region of the GUS coding sequence. Based on this concurrence, we propose a link between the DNA-based transgene methylation and the RNA-based gene silencing process.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Plant Mol Biol. 1990 Dec;15(6):851-64 - PubMed
    1. Science. 1994 Feb 11;263(5148):802-5 - PubMed
    1. Mol Gen Genet. 1990 Dec;224(3):477-81 - PubMed
    1. Plant J. 1993 Jul;4(1):89-100 - PubMed
    1. Plant Cell. 1990 Apr;2(4):279-289 - PubMed

LinkOut - more resources