GTP-yeast actin
- PMID: 12191996
- DOI: 10.1074/jbc.M204025200
GTP-yeast actin
Abstract
Because of the apparently greater conformational flexibility of yeast versus muscle actin and the ability of other members in the actin protein superfamily to efficiently use both ATP and GTP, we assessed the ability of yeast actin to function with GTP. Etheno-ATP exchange studies showed that the binding of GTP to yeast actin is about 1/9 as tight as that of ATP in contrast to the 1/1,240 ratio for muscle actin. Proteolysis of GTP-bound G-yeast actin suggests that the conformation of subdomain 2 is very much like that of ATP-bound actin, but CD studies show that GTP-bound actin is less thermostable than ATP-bound actin. GTP-actin polymerizes with an apparent critical concentration of 1.5 microm, higher than that of ATP-actin (0.3 microm) although filament structures observed by electron microscopy were similar. Yeast actin hydrolyzes GTP in a polymerization-dependent manner, and GTP-bound F-actin decorates with the myosin S1. Conversion of Phe(306) in the nucleotide binding site to the Tyr found in muscle actin raised the nucleotide discrimination ratio from the 1/9 of wild-type actin to 1/125. This result agrees with modeling that predicts that removal of the Tyr hydroxyl will create a space for the C2 amino group of the GTP guanine.
Similar articles
-
Control of the ability of profilin to bind and facilitate nucleotide exchange from G-actin.J Biol Chem. 2008 Apr 4;283(14):9444-53. doi: 10.1074/jbc.M709806200. Epub 2008 Jan 27. J Biol Chem. 2008. PMID: 18223293 Free PMC article.
-
Actin polymerization: regulation by divalent metal ion and nucleotide binding, ATP hydrolysis and binding of myosin.Adv Exp Med Biol. 1994;358:71-81. doi: 10.1007/978-1-4615-2578-3_7. Adv Exp Med Biol. 1994. PMID: 7801813 Review.
-
Modulation of yeast F-actin structure by a mutation in the nucleotide-binding cleft.J Mol Biol. 1997 Aug 15;271(2):235-43. doi: 10.1006/jmbi.1997.1163. J Mol Biol. 1997. PMID: 9268655
-
Structural evidence for the roles of divalent cations in actin polymerization and activation of ATP hydrolysis.Proc Natl Acad Sci U S A. 2018 Oct 9;115(41):10345-10350. doi: 10.1073/pnas.1806394115. Epub 2018 Sep 25. Proc Natl Acad Sci U S A. 2018. PMID: 30254171 Free PMC article.
-
Actin-bound nucleotide/divalent cation interactions.Adv Exp Med Biol. 1994;358:35-49. doi: 10.1007/978-1-4615-2578-3_4. Adv Exp Med Biol. 1994. PMID: 7801810 Review.
Cited by
-
Control of the ability of profilin to bind and facilitate nucleotide exchange from G-actin.J Biol Chem. 2008 Apr 4;283(14):9444-53. doi: 10.1074/jbc.M709806200. Epub 2008 Jan 27. J Biol Chem. 2008. PMID: 18223293 Free PMC article.
-
The bacterial actin MamK: in vitro assembly behavior and filament architecture.J Biol Chem. 2013 Feb 8;288(6):4265-77. doi: 10.1074/jbc.M112.417030. Epub 2012 Nov 30. J Biol Chem. 2013. PMID: 23204522 Free PMC article.
-
Dilated cardiomyopathy-associated skeletal muscle actin (ACTA1) mutation R256H disrupts actin structure and function and causes cardiomyocyte hypocontractility.Proc Natl Acad Sci U S A. 2024 Nov 12;121(46):e2405020121. doi: 10.1073/pnas.2405020121. Epub 2024 Nov 6. Proc Natl Acad Sci U S A. 2024. PMID: 39503885
-
GTPase activity, structure, and mechanical properties of filaments assembled from bacterial cytoskeleton protein MreB.J Bacteriol. 2006 Feb;188(3):968-76. doi: 10.1128/JB.188.3.968-976.2006. J Bacteriol. 2006. PMID: 16428401 Free PMC article.
-
Architecture and assembly of a divergent member of the ParM family of bacterial actin-like proteins.J Biol Chem. 2011 Apr 22;286(16):14282-90. doi: 10.1074/jbc.M110.203828. Epub 2011 Feb 21. J Biol Chem. 2011. PMID: 21339292 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous
