doi: 10.1021/ja027007w.
Addition of p-azido-L-phenylalanine to the genetic code of Escherichia coli
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- PMID: 12148987
- DOI: 10.1021/ja027007w
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Addition of p-azido-L-phenylalanine to the genetic code of Escherichia coli
J Am Chem Soc.
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Abstract
We report the selection of a new orthogonal aminoacyl tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in response to the amber codon, TAG. The amino acid is incorporated in good yield with high fidelity and can be used to crosslink interacting proteins.
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