Drosophila homeodomain protein dHb9 directs neuronal fate via crossrepressive and cell-nonautonomous mechanisms
- PMID: 12123607
- DOI: 10.1016/s0896-6273(02)00743-2
Drosophila homeodomain protein dHb9 directs neuronal fate via crossrepressive and cell-nonautonomous mechanisms
Abstract
Here we present the identification and characterization of dHb9, the Drosophila homolog of vertebrate Hb9, which encodes a factor central to motorneuron (MN) development. We show that dHb9 regulates neuronal fate by restricting expression of Lim3 and Even-skipped (Eve), two homeodomain (HD) proteins required for development of distinct neuronal classes. Also, dHb9 and Lim3 are activated independently of each other in a virtually identical population of ventrally and laterally projecting MNs. Surprisingly, dHb9 represses Lim3 cell nonautonomously in a subset of dorsally projecting MNs, revealing a novel role for intercellular signaling in the establishment of neuronal fate in Drosophila. Lastly, we provide evidence that dHb9 and Eve regulate each other's expression through Groucho-dependent crossrepression. This mutually antagonistic relationship bears similarity to the crossrepressive relationships between pairs of HD proteins that pattern the vertebrate neural tube.
Similar articles
-
Genetic specification of axonal arbors: atonal regulates robo3 to position terminal branches in the Drosophila nervous system.Neuron. 2003 Jan 9;37(1):41-51. doi: 10.1016/s0896-6273(02)01131-5. Neuron. 2003. PMID: 12526771
-
Neuromancer1 and Neuromancer2 regulate cell fate specification in the developing embryonic CNS of Drosophila melanogaster.Dev Biol. 2009 Jan 1;325(1):138-50. doi: 10.1016/j.ydbio.2008.10.006. Epub 2008 Nov 1. Dev Biol. 2009. PMID: 19013145 Free PMC article.
-
Cooperative recruitment of Yan via a high-affinity ETS supersite organizes repression to confer specificity and robustness to cardiac cell fate specification.Genes Dev. 2018 Mar 1;32(5-6):389-401. doi: 10.1101/gad.307132.117. Epub 2018 Mar 13. Genes Dev. 2018. PMID: 29535190 Free PMC article.
-
Vertebrate hairy and Enhancer of split related proteins: transcriptional repressors regulating cellular differentiation and embryonic patterning.Oncogene. 2001 Dec 20;20(58):8342-57. doi: 10.1038/sj.onc.1205094. Oncogene. 2001. PMID: 11840327 Review.
-
40 years of homeodomain transcription factors in the Drosophila nervous system.Development. 2024 Jun 1;151(11):dev202910. doi: 10.1242/dev.202910. Epub 2024 May 31. Development. 2024. PMID: 38819456 Review.
Cited by
-
Single-cell RNA sequencing of motoneurons identifies regulators of synaptic wiring in Drosophila embryos.Mol Syst Biol. 2022 Mar;18(3):e10255. doi: 10.15252/msb.202110255. Mol Syst Biol. 2022. PMID: 35225419 Free PMC article.
-
Dissection of the Regulatory Elements of the Complex Expression Pattern of Puckered, a Dual-Specificity JNK Phosphatase.Int J Mol Sci. 2021 Nov 11;22(22):12205. doi: 10.3390/ijms222212205. Int J Mol Sci. 2021. PMID: 34830088 Free PMC article.
-
Positional cues in the Drosophila nerve cord: semaphorins pattern the dorso-ventral axis.PLoS Biol. 2009 Jun 16;7(6):e1000135. doi: 10.1371/journal.pbio.1000135. Epub 2009 Jun 23. PLoS Biol. 2009. PMID: 19547742 Free PMC article.
-
UNC-4 antagonizes Wnt signaling to regulate synaptic choice in the C. elegans motor circuit.Development. 2012 Jun;139(12):2234-45. doi: 10.1242/dev.075184. Development. 2012. PMID: 22619391 Free PMC article.
-
Regulation of locomotion and motoneuron trajectory selection and targeting by the Drosophila homolog of Olig family transcription factors.Dev Biol. 2012 Sep 15;369(2):261-76. doi: 10.1016/j.ydbio.2012.06.027. Epub 2012 Jul 14. Dev Biol. 2012. PMID: 22796650 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
