Chemical synthesis of poliovirus cDNA: generation of infectious virus in the absence of natural template
- PMID: 12114528
- DOI: 10.1126/science.1072266
Chemical synthesis of poliovirus cDNA: generation of infectious virus in the absence of natural template
Abstract
Full-length poliovirus complementary DNA (cDNA) was synthesized by assembling oligonucleotides of plus and minus strand polarity. The synthetic poliovirus cDNA was transcribed by RNA polymerase into viral RNA, which translated and replicated in a cell-free extract, resulting in the de novo synthesis of infectious poliovirus. Experiments in tissue culture using neutralizing antibodies and CD155 receptor-specific antibodies and neurovirulence tests in CD155 transgenic mice confirmed that the synthetic virus had biochemical and pathogenic characteristics of poliovirus. Our results show that it is possible to synthesize an infectious agent by in vitro chemical-biochemical means solely by following instructions from a written sequence.
Comment in
-
Virology. Active poliovirus baked from scratch.Science. 2002 Jul 12;297(5579):174-5. doi: 10.1126/science.297.5579.174b. Science. 2002. PMID: 12114601 No abstract available.
-
A not-so-cheap stunt.Science. 2002 Aug 2;297(5582):769-70; author reply 769-70. doi: 10.1126/science.297.5582.769b. Science. 2002. PMID: 12162317 No abstract available.
Similar articles
-
In vitro synthesis of poliovirus.Dev Biol Stand. 1993;78:39-53. Dev Biol Stand. 1993. PMID: 8388831
-
Production of infectious poliovirus from synthetic viral genomes.Methods Mol Biol. 2012;852:181-93. doi: 10.1007/978-1-61779-564-0_14. Methods Mol Biol. 2012. PMID: 22328434
-
Molecular biology and cell-free synthesis of poliovirus.Biologicals. 1993 Dec;21(4):349-56. doi: 10.1006/biol.1993.1095. Biologicals. 1993. PMID: 8024750 Review.
-
Studies on neurovirulence in poliovirus-sensitive transgenic mice and cynomolgus monkeys for the different temperature-sensitive viruses derived from the Sabin type 3 virus.Virology. 1995 Jun 20;210(1):160-6. doi: 10.1006/viro.1995.1327. Virology. 1995. PMID: 7793068
-
One hundred years of poliovirus pathogenesis.Virology. 2006 Jan 5;344(1):9-16. doi: 10.1016/j.virol.2005.09.015. Virology. 2006. PMID: 16364730 Review.
Cited by
-
Enhancing Gene Synthesis Security: An Updated Framework for Synthetic Nucleic Acid Screening and the Responsible Use of Synthetic Biological Materials.Appl Biosaf. 2024 Jun 20;29(2):63-70. doi: 10.1089/apb.2023.0036. eCollection 2024 Jun. Appl Biosaf. 2024. PMID: 39144102 Review.
-
Biosafety, biosecurity, and bioethics.Monash Bioeth Rev. 2024 Jun;42(1):137-167. doi: 10.1007/s40592-024-00204-3. Epub 2024 Jul 30. Monash Bioeth Rev. 2024. PMID: 39078602 Free PMC article. Review.
-
Possible Paths to Measles Eradication: Conceptual Frameworks, Strategies, and Tactics.Vaccines (Basel). 2024 Jul 22;12(7):814. doi: 10.3390/vaccines12070814. Vaccines (Basel). 2024. PMID: 39066451 Free PMC article. Review.
-
Designing a synthetic moss genome using GenoDesigner.Nat Plants. 2024 Jun;10(6):848-856. doi: 10.1038/s41477-024-01693-0. Epub 2024 Jun 3. Nat Plants. 2024. PMID: 38831044 Review.
-
Self-Replicating RNA Derived from the Genomes of Positive-Strand RNA Viruses.Methods Mol Biol. 2024;2786:25-49. doi: 10.1007/978-1-0716-3770-8_2. Methods Mol Biol. 2024. PMID: 38814389
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials