Characterization of L-glutamine transport by a human neuroblastoma cell line
- PMID: 11997238
- DOI: 10.1152/ajpcell.00324.2001
Characterization of L-glutamine transport by a human neuroblastoma cell line
Abstract
This study characterized the Na+-dependent transport of L-glutamine by a human neuroblastoma cell line, SK-N-SH. The Na+-dependent component represented >95% of the total glutamine uptake. Kinetic studies showed a single saturable high-affinity carrier with a Michaelis constant (K(m)) of 163 +/- 23 microM and a maximum transport velocity (Vmax) of 13,713 +/- 803 pmol x mg protein(-1) x min(-1). Glutamine uptake was markedly inhibited in the presence of L-alanine, L-asparagine, and L-serine. Li+ did not substitute for Na+. These data show that L-glutamine is predominantly taken up through system ASC. Glutamine deprivation resulted in the decrease of glutamine transport by a mechanism that decreased Vmax without affecting K(m). The expression of the system ASC subtype ASCT2 decreased in the glutamine-deprived group, whereas glutamine deprivation did not induce changes in system ASC subtype ASCT1 mRNA expression. Adaptive increases in Na+-dependent glutamate, Na+-dependent 2-(methylamino)isobutyric acid, and Na+-independent leucine transport were observed under glutamine-deprived conditions, which were completely blocked by actinomycin D and cycloheximide. These mechanisms may allow cells to survive and even grow under nutrient-deprived conditions.
Similar articles
-
Glutamine transport in C6 glioma cells: substrate specificity and modulation in a glutamine deprived culture medium.J Neurosci Res. 2001 Dec 1;66(5):959-66. doi: 10.1002/jnr.10047. J Neurosci Res. 2001. PMID: 11746424
-
Insulin-like growth factor-I stimulates amino acid transport in a glutamine-deprived human neuroblastoma cell line.Biochim Biophys Acta. 2001 Feb 16;1525(1-2):118-24. doi: 10.1016/s0304-4165(00)00178-1. Biochim Biophys Acta. 2001. PMID: 11342260
-
Amino acid uptake and regulation in multicellular hepatoma spheroids.J Surg Res. 2000 Jun 1;91(1):15-25. doi: 10.1006/jsre.2000.5888. J Surg Res. 2000. PMID: 10816344
-
The l-isomer-selective transport of aspartic acid is mediated by ASCT2 at the blood-brain barrier.J Neurochem. 2003 Nov;87(4):891-901. doi: 10.1046/j.1471-4159.2003.02063.x. J Neurochem. 2003. PMID: 14622120
-
Hypoxia upregulates amino acid transport in a human neuroblastoma cell line.J Pediatr Surg. 2007 Apr;42(4):608-12. doi: 10.1016/j.jpedsurg.2006.12.010. J Pediatr Surg. 2007. PMID: 17448754
Cited by
-
An anti-ASCT2 monoclonal antibody suppresses gastric cancer growth by inducing oxidative stress and antibody dependent cellular toxicity in preclinical models.Am J Cancer Res. 2018 Aug 1;8(8):1499-1513. eCollection 2018. Am J Cancer Res. 2018. PMID: 30210919 Free PMC article.
-
Metabolic Evaluation of MYCN-Amplified Neuroblastoma by 4-[18F]FGln PET Imaging.Mol Imaging Biol. 2019 Dec;21(6):1117-1126. doi: 10.1007/s11307-019-01330-9. Mol Imaging Biol. 2019. PMID: 30850970
-
Membrane transporters for the special amino acid glutamine: structure/function relationships and relevance to human health.Front Chem. 2014 Aug 11;2:61. doi: 10.3389/fchem.2014.00061. eCollection 2014. Front Chem. 2014. PMID: 25157349 Free PMC article. Review.
-
Targeting Glutamine Induces Apoptosis: A Cancer Therapy Approach.Int J Mol Sci. 2015 Sep 22;16(9):22830-55. doi: 10.3390/ijms160922830. Int J Mol Sci. 2015. PMID: 26402672 Free PMC article. Review.
-
Glutamine synthetase facilitates cancer cells to recover from irradiation-induced G2/M arrest.Cancer Biol Ther. 2020;21(1):43-51. doi: 10.1080/15384047.2019.1665394. Epub 2019 Sep 17. Cancer Biol Ther. 2020. PMID: 31526079 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials
Miscellaneous
