Two isoforms of the early E74 gene, an Ets transcription factor homologue, are implicated in the ecdysteroid hierarchy governing vitellogenesis of the mosquito, Aedes aegypti
- PMID: 11997188
- DOI: 10.1016/s0303-7207(01)00726-2
Two isoforms of the early E74 gene, an Ets transcription factor homologue, are implicated in the ecdysteroid hierarchy governing vitellogenesis of the mosquito, Aedes aegypti
Abstract
In the anautogenous mosquito, Aedes aegypti, vitellogenesis is under the strict control of 20-hydroxyecdysone (20E), which is produced via a blood meal-activated hormonal cascade. Several genes of the ecdysteroid-regulatory hierarchy are conserved between vitellogenesis in mosquitoes and metamorphosis in Drosophila. We report characterization of two isoforms of the mosquito early E74 gene (AaE74), which have a common C-terminal Ets DNA-binding domain and unique N-termini. They exhibited a high level of identity to Drosophila E74 isoforms A and B and showed structural features typical for Ets transcription factors. Both mosquito E74 isoforms bound to an E74 consensus motif C/AGGAA. In the fat body and ovary, the transcript of AaE74 isoform homologous to Drosophila E74B was induced by a blood meal exhibiting its highest level coinciding with the peak of vitellogenesis. In contrast, the transcript of AaE74 isoform homologous to Drosophila E74A was activated at the termination of vitellogenesis. These findings suggest that AaE74A and AaE74B isoforms play different roles in regulation of vitellogenesis in mosquitoes.
Similar articles
-
The early gene E74B isoform is a transcriptional activator of the ecdysteroid regulatory hierarchy in mosquito vitellogenesis.Mol Cell Endocrinol. 2004 Apr 15;218(1-2):95-105. doi: 10.1016/j.mce.2003.12.014. Mol Cell Endocrinol. 2004. PMID: 15130514
-
The early gene Broad is involved in the ecdysteroid hierarchy governing vitellogenesis of the mosquito Aedes aegypti.J Mol Endocrinol. 2004 Dec;33(3):743-61. doi: 10.1677/jme.1.01531. J Mol Endocrinol. 2004. PMID: 15591032
-
Synergistic action of E74B and ecdysteroid receptor in activating a 20-hydroxyecdysone effector gene.Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15506-11. doi: 10.1073/pnas.0503501102. Epub 2005 Oct 17. Proc Natl Acad Sci U S A. 2005. PMID: 16230625 Free PMC article.
-
Molecular biology of mosquito vitellogenesis: from basic studies to genetic engineering of antipathogen immunity.Insect Biochem Mol Biol. 2002 Oct;32(10):1275-86. doi: 10.1016/s0965-1748(02)00090-5. Insect Biochem Mol Biol. 2002. PMID: 12225918 Review.
-
Sequence and functional properties of Ets genes in the model organism Drosophila.Oncogene. 2000 Dec 18;19(55):6409-16. doi: 10.1038/sj.onc.1204033. Oncogene. 2000. PMID: 11175357 Review.
Cited by
-
Broad-complex Z3 contributes to the ecdysone-mediated transcriptional regulation of the vitellogenin gene in Bombus lantschouensis.PLoS One. 2018 Nov 15;13(11):e0207275. doi: 10.1371/journal.pone.0207275. eCollection 2018. PLoS One. 2018. PMID: 30440013 Free PMC article.
-
The Function of Nilaparvata lugens (Hemiptera: Delphacidae) E74 and Its Interaction With βFtz-F1.J Insect Sci. 2022 May 1;22(3):15. doi: 10.1093/jisesa/ieac041. J Insect Sci. 2022. PMID: 35738261 Free PMC article.
-
Posttranscriptional control of the competence factor betaFTZ-F1 by juvenile hormone in the mosquito Aedes aegypti.Proc Natl Acad Sci U S A. 2003 Nov 11;100(23):13338-43. doi: 10.1073/pnas.2234416100. Epub 2003 Oct 30. Proc Natl Acad Sci U S A. 2003. PMID: 14593204 Free PMC article.
-
The small GTPase Rheb is a key component linking amino acid signaling and TOR in the nutritional pathway that controls mosquito egg development.Insect Biochem Mol Biol. 2011 Jan;41(1):62-9. doi: 10.1016/j.ibmb.2010.10.001. Epub 2010 Oct 28. Insect Biochem Mol Biol. 2011. PMID: 21035549 Free PMC article.
-
Changes in the Gene Expression Profiles of the Hypopharyngeal Gland of Worker Honeybees in Association with Worker Behavior and Hormonal Factors.PLoS One. 2015 Jun 17;10(6):e0130206. doi: 10.1371/journal.pone.0130206. eCollection 2015. PLoS One. 2015. PMID: 26083737 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
