doi: 10.1126/science.1068768.
Subnuclear compartmentalization of immunoglobulin loci during lymphocyte development
Affiliations
- PMID: 11935030
- DOI: 10.1126/science.1068768
Item in Clipboard
Subnuclear compartmentalization of immunoglobulin loci during lymphocyte development
Science.
.
Abstract
Immunoglobulin (Ig) loci are selectively activated for transcription and rearrangement during B lymphocyte development. Using fluorescence in situ hybridization, we show that Ig heavy (H) and Igkappa loci are preferentially positioned at the nuclear periphery in hematopoietic progenitors and pro-T cells but are centrally configured in pro-B nuclei. The inactive loci at the periphery do not associate with centromeric heterochromatin. Upon localization away from the nuclear periphery in pro-B cells, the IgH locus appears to undergo large-scale compaction. We suggest that subnuclear positioning represents a novel means of regulating transcription and recombination of IgH and Igkappa loci during lymphocyte development.
Similar articles
-
Nonequivalent nuclear location of immunoglobulin alleles in B lymphocytes.Nat Immunol. 2001 Sep;2(9):848-54. doi: 10.1038/ni0901-848. Nat Immunol. 2001. PMID: 11526401
-
Visualization of looping involving the immunoglobulin heavy-chain locus in developing B cells.Genes Dev. 2005 Feb 1;19(3):322-7. doi: 10.1101/gad.1254305. Genes Dev. 2005. PMID: 15687256 Free PMC article.
-
Kappa and lambda rearrangement occur simultaneously in transformed pre-B cells.J Immunol. 1997 Dec 15;159(12):6061-9. J Immunol. 1997. PMID: 9550405
-
VH replacement in mice and humans.Trends Immunol. 2007 Mar;28(3):132-7. doi: 10.1016/j.it.2007.01.003. Epub 2007 Jan 26. Trends Immunol. 2007. PMID: 17258935 Review.
-
Diversity of immunoglobulin structural gene loci. The special case of the mouse lambda locus.Surv Immunol Res. 1983;2(1):43-51. Surv Immunol Res. 1983. PMID: 6417754 Review. No abstract available.
Cited by
-
Osmotic challenge drives rapid and reversible chromatin condensation in chondrocytes.Biophys J. 2013 Feb 19;104(4):759-69. doi: 10.1016/j.bpj.2013.01.006. Biophys J. 2013. PMID: 23442954 Free PMC article.
-
Heterochromatin as an Important Driver of Genome Organization.Front Cell Dev Biol. 2020 Sep 18;8:579137. doi: 10.3389/fcell.2020.579137. eCollection 2020. Front Cell Dev Biol. 2020. PMID: 33072761 Free PMC article. Review.
-
A negative feedback loop at the nuclear periphery regulates GAL gene expression.Mol Biol Cell. 2012 Apr;23(7):1367-75. doi: 10.1091/mbc.E11-06-0547. Epub 2012 Feb 9. Mol Biol Cell. 2012. PMID: 22323286 Free PMC article.
-
Non-random organization of the Biomphalaria glabrata genome in interphase Bge cells and the spatial repositioning of activated genes in cells co-cultured with Schistosoma mansoni.Int J Parasitol. 2011 Jan;41(1):61-70. doi: 10.1016/j.ijpara.2010.07.015. Epub 2010 Sep 16. Int J Parasitol. 2011. PMID: 20849859 Free PMC article.
-
Engineering 3D genome organization.Cell Res. 2019 Jan;29(1):1-3. doi: 10.1038/s41422-018-0116-5. Cell Res. 2019. PMID: 30514898 Free PMC article. No abstract available.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
