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. 2002 Feb 1;293(1):141-50.
doi: 10.1006/viro.2001.1270.

Autogenous translational inhibition of core protein: implication for switch from translation to RNA replication in hepatitis C virus

Jing Zhang  1 , Osamu Yamada, Hiroshi Yoshida, Takahiro Iwai, Hiromasa Araki
Affiliations
Free article

Autogenous translational inhibition of core protein: implication for switch from translation to RNA replication in hepatitis C virus

Jing Zhang et al. Virology. .
Free article

Abstract

Positive-stranded viruses use the genomic RNA as a common template for translation and RNA replication which proceed in inverse direction; a certain regulatory mechanism for translation control is probably required to coordinate these two antagonistic processes. Hepatitis C virus (HCV) core protein is a good candidate that might play a role in such a regulation. In this study, we further investigated whether HCV core protein modulates internal ribosome entry site (IRES)-directed translation. The inclusion of the core-coding sequence significantly suppressed translation initiated by HCV IRES in monocistronic and bicistronic reporter systems. The region mainly responsible for this inhibition was mapped to nt 441-473 of the core-coding sequence. This suppression was eliminated by frameshift mutations introduced into this region, suggesting that it is the core protein expressed in cis, rather than the core-coding nucleotide sequence that negatively modulates the efficiency of HCV IRES-dependent translation. Furthermore, the core protein provided in trans also specifically decreased the IRES activity in directing cap-independent translation both in transfected cells and in cell-free translation study. Consistently, a gel mobility shift assay showed a specific interaction between the core protein and HCV IRES-containing RNA transcript. These findings suggest that HCV core protein may down-regulate the cap-independent translation as a regulatory mechanism required for initiation of transcription.

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