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. 2002 Feb;10(2):127-9.
doi: 10.1016/s0969-2126(02)00710-4.

Cytosines do it, thymines do it, even pseudouridines do it--base flipping by an enzyme that acts on RNA

Xiaodong Cheng  1 , Robert M Blumenthal
Affiliations

Cytosines do it, thymines do it, even pseudouridines do it--base flipping by an enzyme that acts on RNA

Xiaodong Cheng et al. Structure. 2002 Feb.

Abstract

In the December 28, 2001 issue of Cell, Hoang and Ferré-D'Amaré report the structure of a tRNA pseudouridine synthase, showing the target uridine flipped out from the tRNA and confirming that base flipping is not limited to enzymes that act on DNA.

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Figures

Figure
Figure. Examples of RNA Base Flipping
(A) 30S ribosomal subunit: flipped-out A1492 and A1493 from helix 44 (in cyan) of 16S RNA by binding of paromomycin (left, seen in the orange difference density) and initiation factor IF1 (right, in purple). The protein S12 is in yellow. (B) Restrictocin-RNA complex (Protein Data Bank code 1JBR). The RNA is presented as a magenta stick model, with the flipped nucleotide in green. The enzyme is represented as silver ribbons. (C) TruB-tRNA complex (Protein Data Bank code 1K8W). The flipped nucleotides are in green (U55) and cyan (C56 and G57). The amino acid His43 in dark blue stacks with U54:A58 pair. (D) Stick model of yeast tRNAPhe (Protein Data Bank code 1EHZ), with the stem loop in magenta, U55 in green, C56 and G57 in cyan, and G18 of D loop in dark blue.
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References

    1. Klimasauskas S, Kumar S, Roberts RJ, Cheng X. HhaI methyltransferase flips its target base out of the DNA helix. Cell. 1994;76:357–369. - PubMed
    1. Roberts RJ, Cheng X. Base flipping. Annu Rev Biochem. 1998;67:181–198. - PubMed
    1. Rupert PB, Ferré-D’Amaré AR. Crystal structure of a hairpin ribozyme-inhibitor complex with implications for catalysis. Nature. 2001;410:780–786. - PubMed
    1. Andersen AA, Collins RA. Rearrangement of a stable RNA secondary structure during VS ribozyme catalysis. Mol Cell. 2000;5:469–478. - PubMed
    1. Carter AP, Clemons WM, Brodersen DE, Morgan-Warren RJ, Wimberly BT, Ramakrishnan V. Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics. Nature. 2000;407:340–348. - PubMed

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