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. 2002 Mar;282(3):E733-8.
doi: 10.1152/ajpendo.2002.282.3.E733.

Identification of a novel glucose transporter-like protein-GLUT-12

Suzanne Rogers  1 , Maria L Macheda, Susan E Docherty, Maynard D Carty, Michael A Henderson, Walter C Soeller, E Michael Gibbs, David E James, James D Best
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Free article

Identification of a novel glucose transporter-like protein-GLUT-12

Suzanne Rogers et al. Am J Physiol Endocrinol Metab. 2002 Mar.
Free article

Abstract

Facilitative glucose transporters exhibit variable hexose affinity and tissue-specific expression. These characteristics contribute to specialized metabolic properties of cells. Here we describe the characterization of a novel glucose transporter-like molecule, GLUT-12. GLUT-12 was identified in MCF-7 breast cancer cells by homology to the insulin-regulatable glucose transporter GLUT-4. The GLUT-12 cDNA encodes 617 amino acids, which possess features essential for sugar transport. Di-leucine motifs are present in NH(2) and COOH termini at positions similar to the GLUT-4 FQQI and LL targeting motifs. GLUT-12 exhibits 29% amino acid identity with GLUT-4 and 40% to the recently described GLUT-10. Like GLUT-10, a large extracellular domain is predicted between transmembrane domains 9 and 10. Genomic organization of GLUT-12 is highly conserved with GLUT-10 but distinct from GLUTs 1-5. Immunofluorescence showed that, in the absence of insulin, GLUT-12 is localized to the perinuclear region in MCF-7 cells. Immunoblotting demonstrated GLUT-12 expression in skeletal muscle, adipose tissue, and small intestine. Thus GLUT-12 is potentially part of a second insulin-responsive glucose transport system.

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