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. 2002 Mar 8;277(10):8033-40.
doi: 10.1074/jbc.M108552200. Epub 2002 Jan 2.

c-Cbl binding and ubiquitin-dependent lysosomal degradation of membrane-associated Notch1

Birgit M Jehn  1 , Irina Dittert, Stephanie Beyer, Klaus von der Mark, Wolfgang Bielke
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Free article

c-Cbl binding and ubiquitin-dependent lysosomal degradation of membrane-associated Notch1

Birgit M Jehn et al. J Biol Chem. .
Free article

Abstract

Regulation of Notch1 activity is critical for cell fate decisions and differentiation of skeletal myoblasts. We have employed the skeletal myoblast cell line C2C12 to study posttranslational regulation of Notch1 protein levels during myogenesis. Although the major degradation pathway of the activated intracellular Notch1 fragment appears to involve ubiquitination and degradation by the 26 S proteasome, we provide evidence for an alternative catalytic pathway where the endogenous, transmembrane form of Notch1 is targeted to the lysosomal compartment. Immunoprecipitation analysis revealed ubiquitin-dependent accumulation of transmembrane Notch1 protein after treatment with the lysosomal inhibitor chloroquine but not after treatment with various proteasome inhibitors. This finding was supported by the observation that the transmembrane form of Notch1 was tyrosine-phosphorylated and specifically coprecipitated with the ubiquitin ligase c-Cbl. Our data suggest a regulatory mechanism down-regulating Notch1 protein levels already at the cellular surface, possibly with consequences for Notch-dependent signal transduction during terminal differentiation processes.

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