Real-time single-molecule imaging of the infection pathway of an adeno-associated virus
- PMID: 11729319
- DOI: 10.1126/science.1064103
Real-time single-molecule imaging of the infection pathway of an adeno-associated virus
Abstract
We describe a method, based on single-molecule imaging, that allows the real-time visualization of the infection pathway of single viruses in living cells, each labeled with only one fluorescent dye molecule. The tracking of single viruses removes ensemble averaging. Diffusion trajectories with high spatial and time resolution show various modes of motion of adeno-associated viruses (AAV) during their infection pathway into living HeLa cells: (i) consecutive virus touching at the cell surface and fast endocytosis; (ii) free and anomalous diffusion of the endosome and the virus in the cytoplasm and the nucleus; and (iii) directed motion by motor proteins in the cytoplasm and in nuclear tubular structures. The real-time visualization of the infection pathway of single AAVs shows a much faster infection than was generally observed so far.
Comment in
-
Molecular imaging. Virus infects cell: live and uncut.Science. 2001 Nov 30;294(5548):1803. doi: 10.1126/science.294.5548.1803a. Science. 2001. PMID: 11729272 No abstract available.
Similar articles
-
Molecular imaging. Virus infects cell: live and uncut.Science. 2001 Nov 30;294(5548):1803. doi: 10.1126/science.294.5548.1803a. Science. 2001. PMID: 11729272 No abstract available.
-
Quantitative four-dimensional tracking of cytoplasmic and nuclear HIV-1 complexes.Nat Methods. 2006 Oct;3(10):817-24. doi: 10.1038/nmeth928. Nat Methods. 2006. PMID: 16990814
-
Infectious entry pathway of adeno-associated virus and adeno-associated virus vectors.J Virol. 2000 Mar;74(6):2777-85. doi: 10.1128/jvi.74.6.2777-2785.2000. J Virol. 2000. PMID: 10684294 Free PMC article.
-
Characterization of simian virus 40 on its infectious entry pathway in cells using fluorescence correlation spectroscopy.Biochem Soc Trans. 2004 Nov;32(Pt 5):746-9. doi: 10.1042/BST0320746. Biochem Soc Trans. 2004. PMID: 15494004 Review.
-
Quantitative microscopy: protein dynamics and membrane organisation.Traffic. 2009 Aug;10(8):962-71. doi: 10.1111/j.1600-0854.2009.00908.x. Epub 2009 Apr 4. Traffic. 2009. PMID: 19416480 Review.
Cited by
-
Chemical approaches to probe and engineer AAV vectors.Nanoscale. 2024 Jul 25;16(29):13820-13833. doi: 10.1039/d4nr01300j. Nanoscale. 2024. PMID: 38978480 Free PMC article. Review.
-
Analysis of the impact of pluronic acid on the thermal stability and infectivity of AAV6.2FF.BMC Biotechnol. 2024 Apr 25;24(1):22. doi: 10.1186/s12896-024-00853-6. BMC Biotechnol. 2024. PMID: 38664752 Free PMC article.
-
A Spatial Interpolation Approach to Assign Magnetic Resonance Imaging-Derived Material Properties for Finite Element Models of Adeno-Associated Virus Infusion Into a Recurrent Brain Tumor.J Biomech Eng. 2024 Oct 1;146(10):101001. doi: 10.1115/1.4064966. J Biomech Eng. 2024. PMID: 38581376
-
Natural Adeno-Associated Virus Serotypes and Engineered Adeno-Associated Virus Capsid Variants: Tropism Differences and Mechanistic Insights.Viruses. 2024 Mar 12;16(3):442. doi: 10.3390/v16030442. Viruses. 2024. PMID: 38543807 Free PMC article. Review.
-
Beyond microtubules: The cellular environment at the endoplasmic reticulum attracts proteins to the nucleus, enabling nuclear transport.iScience. 2024 Feb 15;27(3):109235. doi: 10.1016/j.isci.2024.109235. eCollection 2024 Mar 15. iScience. 2024. PMID: 38439967 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources