HIV-1 and Ebola virus encode small peptide motifs that recruit Tsg101 to sites of particle assembly to facilitate egress
- PMID: 11726971
- DOI: 10.1038/nm1201-1313
HIV-1 and Ebola virus encode small peptide motifs that recruit Tsg101 to sites of particle assembly to facilitate egress
Abstract
Retroviral Gag proteins encode sequences, termed late domains, which facilitate the final stages of particle budding from the plasma membrane. We report here that interactions between Tsg101, a factor involved in endosomal protein sorting, and short peptide motifs in the HIV-1 Gag late domain and Ebola virus matrix (EbVp40) proteins are essential for efficient egress of HIV-1 virions and Ebola virus-like particles. EbVp40 recruits Tsg101 to sites of particle assembly and a short, EbVp40-derived Tsg101-binding peptide sequence can functionally substitute for the HIV-1 Gag late domain. Notably, recruitment of Tsg101 to assembling virions restores budding competence to a late-domain-defective HIV-1 in the complete absence of viral late domain. These studies define an essential virus-host interaction that is conserved in two unrelated viruses. Because the Tsg101 is recruited by small, conserved viral sequence motifs, agents that mimic these structures are potential inhibitors of the replication of these lethal human pathogens.
Comment in
-
HIV-1 and Ebola virus: the getaway driver nabbed.Nat Med. 2001 Dec;7(12):1278-80. doi: 10.1038/nm1201-1278. Nat Med. 2001. PMID: 11726960 No abstract available.
Similar articles
-
Overexpression of the N-terminal domain of TSG101 inhibits HIV-1 budding by blocking late domain function.Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):955-60. doi: 10.1073/pnas.032511899. Proc Natl Acad Sci U S A. 2002. PMID: 11805336 Free PMC article.
-
Structure of the Tsg101 UEV domain in complex with the PTAP motif of the HIV-1 p6 protein.Nat Struct Biol. 2002 Nov;9(11):812-7. doi: 10.1038/nsb856. Nat Struct Biol. 2002. PMID: 12379843
-
HIV Gag mimics the Tsg101-recruiting activity of the human Hrs protein.J Cell Biol. 2003 Aug 4;162(3):425-34. doi: 10.1083/jcb.200302138. J Cell Biol. 2003. PMID: 12900394 Free PMC article.
-
[The action of TSG101 on HIV-1 budding and related inhibitors].Yao Xue Xue Bao. 2008 Dec;43(12):1165-70. Yao Xue Xue Bao. 2008. PMID: 19244744 Review. Chinese.
-
HIV-1 gag proteins: diverse functions in the virus life cycle.Virology. 1998 Nov 10;251(1):1-15. doi: 10.1006/viro.1998.9398. Virology. 1998. PMID: 9813197 Review.
Cited by
-
High-throughput virtual screening and docking studies of matrix protein vp40 of ebola virus.Bioinformation. 2013;9(6):286-92. doi: 10.6026/97320630009286. Epub 2013 Mar 19. Bioinformation. 2013. PMID: 23559747 Free PMC article.
-
Formation and release of arrestin domain-containing protein 1-mediated microvesicles (ARMMs) at plasma membrane by recruitment of TSG101 protein.Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4146-51. doi: 10.1073/pnas.1200448109. Epub 2012 Feb 6. Proc Natl Acad Sci U S A. 2012. PMID: 22315426 Free PMC article.
-
The clathrin adaptor complex AP-1 binds HIV-1 and MLV Gag and facilitates their budding.Mol Biol Cell. 2007 Aug;18(8):3193-203. doi: 10.1091/mbc.e06-12-1147. Epub 2007 May 30. Mol Biol Cell. 2007. PMID: 17538020 Free PMC article.
-
Extracellular vesicles and nanoparticles: emerging complexities.Trends Cell Biol. 2023 Aug;33(8):667-681. doi: 10.1016/j.tcb.2023.01.002. Epub 2023 Feb 1. Trends Cell Biol. 2023. PMID: 36737375 Free PMC article. Review.
-
Paramyxovirus assembly and budding: building particles that transmit infections.Int J Biochem Cell Biol. 2010 Sep;42(9):1416-29. doi: 10.1016/j.biocel.2010.04.005. Epub 2010 Apr 14. Int J Biochem Cell Biol. 2010. PMID: 20398786 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
