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Comparative Study
. 2001 Nov;8(6):1150-5.
doi: 10.1128/CDLI.8.6.1150-1155.2001.

Use of recombinant envelope proteins for serological diagnosis of Dengue virus infection in an immunochromatographic assay

A J Cuzzubbo  1 , T P Endy, A Nisalak, S Kalayanarooj, D W Vaughn, S A Ogata, D E Clements, P L Devine
Affiliations
Comparative Study

Use of recombinant envelope proteins for serological diagnosis of Dengue virus infection in an immunochromatographic assay

A J Cuzzubbo et al. Clin Diagn Lab Immunol. 2001 Nov.

Abstract

An immunochromatographic test that incorporates recombinant antigens (Dengue Duo Rapid Strip Test; PanBio, Brisbane, Australia) has recently become commercially available. This assay is performed in 15 min and detects both immunoglobulin M (IgM) and IgG in a capture format. The four recombinant proteins used represent the N-terminal 80% of the viral envelope glycoproteins of dengue viruses 1, 2, 3, and 4, respectively. The sensitivity and specificity of the recombinant-antigen-based assay were 90 and 86%, respectively. The similar diagnostic performance of these antigens to that of enzyme-linked immunosorbent assays using whole dengue virus suggests that they mimic whole dengue viruses in primary structure and epitope conformation. These results suggest that recombinant proteins can be used in diagnostic assays for dengue to overcome safety issues associated with the use of whole virus.

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Figures

FIG. 1
FIG. 1
Interpretation of the Dengue Duo Rapid Strip Test.
FIG. 2
FIG. 2
Correlation between the ELISA and the rapid test for IgM (a) and IgG (b). The broken lines represent the cutoff units used in the ELISA. The horizontal bars represent the mean ELISA ratio for each rapid score.
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