High-yield selection and extraction of two promoter-defined phenotypes of neural stem cells from the fetal human brain
- PMID: 11533643
- DOI: 10.1038/nbt0901-843
High-yield selection and extraction of two promoter-defined phenotypes of neural stem cells from the fetal human brain
Abstract
Neural stem and precursor cells reside in the ventricular lining of the fetal forebrain, and may provide a cellular substrate for brain repair. To selectively identify and extract these cells, we infected dissociated fetal human brain cells with adenoviruses bearing the gene for green fluorescence protein (GFP), placed under the control of enhancer/promoters for two genes (nestin and musashi1) that are expressed in uncommitted neuroepithelial cells. The cells were then sorted by fluorescence-activated cell sorting (FACS) on the basis of E/nestin- or P/musashi1-driven GFP expression. Both P/musashi1:hGFP- and E/nestin:EGFP-sorted cells were multipotent: limiting dilution with clonal expansion as neurospheres, in tandem with retroviral lineage analysis and xenograft to E17 and P0-2 rat forebrain, revealed that each phenotype was able to both self-renew and co-generate neurons and glia. Thus, fluorescent genes placed under the control of early neural promoters allow neural stem cells to be specifically targeted, isolated, and substantially enriched from the fetal human brain.
Similar articles
-
Promoter-targeted selection and isolation of neural progenitor cells from the adult human ventricular zone.J Neurosci Res. 2000 Feb 1;59(3):321-31. doi: 10.1002/(sici)1097-4547(20000201)59:3<321::aid-jnr5>3.0.co;2-9. J Neurosci Res. 2000. PMID: 10679767
-
Generation of dopaminergic neurons in the adult brain from mesencephalic precursor cells labeled with a nestin-GFP transgene.J Neurosci. 2001 Jun 1;21(11):3895-903. doi: 10.1523/JNEUROSCI.21-11-03895.2001. J Neurosci. 2001. PMID: 11356877 Free PMC article.
-
Direct isolation of committed neuronal progenitor cells from transgenic mice coexpressing spectrally distinct fluorescent proteins regulated by stage-specific neural promoters.J Neurosci Res. 2001 Aug 1;65(3):220-7. doi: 10.1002/jnr.1145. J Neurosci Res. 2001. PMID: 11494356
-
Neural stem and progenitor cells in nestin-GFP transgenic mice.J Comp Neurol. 2004 Feb 9;469(3):311-24. doi: 10.1002/cne.10964. J Comp Neurol. 2004. PMID: 14730584
-
In vitro neurogenesis by progenitor cells isolated from the adult human hippocampus.Nat Med. 2000 Mar;6(3):271-7. doi: 10.1038/73119. Nat Med. 2000. PMID: 10700228
Cited by
-
Molecular and morphological heterogeneity of neural precursors in the mouse neocortical proliferative zones.J Neurosci. 2006 Jan 18;26(3):1045-56. doi: 10.1523/JNEUROSCI.4499-05.2006. J Neurosci. 2006. PMID: 16421324 Free PMC article.
-
Identification of a novel intronic enhancer responsible for the transcriptional regulation of musashi1 in neural stem/progenitor cells.Mol Brain. 2011 Apr 13;4:14. doi: 10.1186/1756-6606-4-14. Mol Brain. 2011. PMID: 21486496 Free PMC article.
-
Cell therapy for multiple sclerosis.Neurotherapeutics. 2011 Oct;8(4):625-42. doi: 10.1007/s13311-011-0073-x. Neurotherapeutics. 2011. PMID: 21904787 Free PMC article. Review.
-
Human Glial Chimeric Mice to Define the Role of Glial Pathology in Human Disease.Methods Mol Biol. 2019;1936:311-331. doi: 10.1007/978-1-4939-9072-6_18. Methods Mol Biol. 2019. PMID: 30820907 Free PMC article. Review.
-
Cell-surface marker signatures for the isolation of neural stem cells, glia and neurons derived from human pluripotent stem cells.PLoS One. 2011 Mar 2;6(3):e17540. doi: 10.1371/journal.pone.0017540. PLoS One. 2011. PMID: 21407814 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
