Evidence that human cardiac myocytes divide after myocardial infarction
- PMID: 11396441
- DOI: 10.1056/NEJM200106073442303
Evidence that human cardiac myocytes divide after myocardial infarction
Expression of concern in
-
Expression of Concern: Beltrami AP et al. Evidence That Human Cardiac Myocytes Divide after Myocardial Infarction. N Engl J Med 2001;344:1750-7 and Quaini F et al. Chimerism of the Transplanted Heart. N Engl J Med 2002;346:5-15.N Engl J Med. 2018 Nov 8;379(19):1870. doi: 10.1056/NEJMe1813801. Epub 2018 Oct 17. N Engl J Med. 2018. PMID: 30332558 No abstract available.
Abstract
Background: The scarring of the heart that results from myocardial infarction has been interpreted as evidence that the heart is composed of myocytes that are unable to divide. However, recent observations have provided evidence of proliferation of myocytes in the adult heart. Therefore, we studied the extent of mitosis among myocytes after myocardial infarction in humans.
Methods: Samples from the border of the infarct and from areas of the myocardium distant from the infarct were obtained from 13 patients who had died 4 to 12 days after infarction. Ten normal hearts were used as controls. Myocytes that had entered the cell cycle in preparation for cell division were measured by labeling of the nuclear antigen Ki-67, which is associated with cell division. The fraction of myocyte nuclei that were undergoing mitosis was determined, and the mitotic index (the ratio of the number of nuclei undergoing mitosis to the number not undergoing mitosis) was calculated. The presence of mitotic spindles, contractile rings, karyokinesis, and cytokinesis was also recorded.
Results: In the infarcted hearts, Ki-67 expression was detected in 4 percent of myocyte nuclei in the regions adjacent to the infarcts and in 1 percent of those in regions distant from the infarcts. The reentry of myocytes into the cell cycle resulted in mitotic indexes of 0.08 percent and 0.03 percent, respectively, in the zones adjacent to and distant from the infarcts. Events characteristic of cell division--the formation of the mitotic spindles, the formation of contractile rings, karyokinesis, and cytokinesis--were identified; these features demonstrated that there was myocyte proliferation after myocardial infarction.
Conclusions: Our results challenge the dogma that the adult heart is a postmitotic organ and indicate that the regeneration of myocytes may be a critical component of the increase in muscle mass of the myocardium.
Comment in
-
High hopes for the heart.N Engl J Med. 2001 Jun 7;344(23):1785-7. doi: 10.1056/NEJM200106073442311. N Engl J Med. 2001. PMID: 11396449 No abstract available.
-
Evidence that human cardiac myocytes divide after myocardial infarction.N Engl J Med. 2001 Oct 11;345(15):1130-1. doi: 10.1056/NEJM200110113451513. N Engl J Med. 2001. PMID: 11596596 No abstract available.
-
Evidence that human cardiac myocytes divide after myocardial infarction.N Engl J Med. 2001 Oct 11;345(15):1130; author reply 1131. N Engl J Med. 2001. PMID: 11596597 No abstract available.
-
Evidence that human cardiac myocytes divide after myocardial infarction.N Engl J Med. 2001 Oct 11;345(15):1131. doi: 10.1056/NEJM200110113451514. N Engl J Med. 2001. PMID: 11596598 No abstract available.
-
Intramyocardial transplantation of endothelial progenitor cells and erythropoietin: a new scope for the treatment of cardiovascular disease.J Surg Res. 2013 Aug;183(2):550-2. doi: 10.1016/j.jss.2012.05.010. Epub 2012 May 23. J Surg Res. 2013. PMID: 22656040 No abstract available.
Similar articles
-
Endomitosis and polyploidization of myocardial cells in the periphery of human acute myocardial infarction.Cardiovasc Res. 2005 Jul 1;67(1):116-23. doi: 10.1016/j.cardiores.2005.02.017. Epub 2005 Mar 31. Cardiovasc Res. 2005. PMID: 15949475
-
High hopes for the heart.N Engl J Med. 2001 Jun 7;344(23):1785-7. doi: 10.1056/NEJM200106073442311. N Engl J Med. 2001. PMID: 11396449 No abstract available.
-
End-stage cardiac failure in humans is coupled with the induction of proliferating cell nuclear antigen and nuclear mitotic division in ventricular myocytes.Circ Res. 1994 Dec;75(6):1050-63. doi: 10.1161/01.res.75.6.1050. Circ Res. 1994. PMID: 7955143
-
Myocyte growth in the failing heart.Surg Clin North Am. 2004 Feb;84(1):161-77. doi: 10.1016/S0039-6109(03)00215-9. Surg Clin North Am. 2004. PMID: 15053188 Review.
-
Myocardial gap junction organization in ischemia and infarction.Microsc Res Tech. 1995 Aug 1;31(5):375-86. doi: 10.1002/jemt.1070310507. Microsc Res Tech. 1995. PMID: 8534899 Review.
Cited by
-
Multipotent stem cells of the heart-do they have therapeutic promise?Front Physiol. 2015 May 8;6:123. doi: 10.3389/fphys.2015.00123. eCollection 2015. Front Physiol. 2015. PMID: 26005421 Free PMC article. Review.
-
Optimised protocols for the identification of the murine cardiac side population.Stem Cell Rev Rep. 2013 Oct;9(5):731-9. doi: 10.1007/s12015-013-9440-9. Stem Cell Rev Rep. 2013. PMID: 23619929 Free PMC article.
-
RNA mimics as therapeutics for cardiac regeneration: a paradigm shift.Mol Ther. 2015 Jun;23(6):984-986. doi: 10.1038/mt.2015.86. Mol Ther. 2015. PMID: 26022627 Free PMC article. No abstract available.
-
Origin of cardiomyocytes in the adult heart.Circ Res. 2015 Jan 2;116(1):150-66. doi: 10.1161/CIRCRESAHA.116.303595. Circ Res. 2015. PMID: 25552694 Free PMC article. Review.
-
Sequestration of p27 within the cytoplasm of cardiac myocytes in chronic ischemic heart disease: pathogenic implications for ischemic cardiomyopathy.Age (Dordr). 2006 Mar;28(1):85-91. doi: 10.1007/s11357-006-9001-0. Epub 2006 May 23. Age (Dordr). 2006. PMID: 23598681 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical