Epidermal growth factor receptor-targeted immunophotodiagnosis and photoimmunotherapy of oral precancer in vivo
- PMID: 11389080
- PMCID: PMC3441050
Epidermal growth factor receptor-targeted immunophotodiagnosis and photoimmunotherapy of oral precancer in vivo
Abstract
Immunophotodiagnosis uses a fluorescence-labeled monoclonal antibody (MAb) that recognizes a tumor-associated antigen to image the fluorescence emitted from the fluorophore-bound MAb that has localized in the tissue. It may be used to diagnose malignant or precancerous lesions, to delineate the margins for tumor resection, or as a feedback mechanism to assess response to treatment. In oral precancer, the epidermal growth factor receptor (EGFR) is overexpressed and could be used as a marker for early detection or as a target for therapy. The goal of this study was to test an anti-EGFR MAb (C225) coupled to either the near-infrared fluorescent dye N,N'-di-carboxypentyl-indodicarbocyanine-5,5'-disulfonic acid for detection or a photochemically active dye (chlorin(e6)) for therapy of early premalignancy in the hamster cheek pouch carcinogenesis model. Fluorescence levels in the carcinogen-treated tissue correlated with the histological stage of the lesions when the C225-N,N'-di-carboxypentyl-indodicarbocyanine-5,5'-disulfonic acid conjugate was used but did not do so with the irrelevant conjugates. Discrete areas of clinically normal mucosa with high fluorescence (hot spots) were subsequently shown by histology to contain dysplastic areas. The best contrast between normal and carcinogen-treated cheek pouches was found at 4-8 days after injection. To test the potential of immunophotodiagnosis as a feedback modality for therapeutic intervention, experiments were conducted with the same MAb conjugated to chlorin(e6) followed by illumination to reduce expression of the EGFR by a photodynamic effect. Subsequent immunophotodiagnosis showed that this treatment led to a significant reduction in fluorescence in the carcinogen-treated cheek pouch compared with nonilluminated areas. This difference between illuminated and dark areas was not seen in the normal cheek pouch. Taken together, the results demonstrate the potential for development of immunophotodiagnosis as a diagnostic tool and as a method of monitoring response to therapy and that the EGFR may be an appropriate target in head and neck cancer.
Figures
Similar articles
-
Novel delivery of Chlorin e6 using anti-EGFR antibody tagged virosomes for fluorescence diagnosis of oral cancer in a hamster cheek pouch model.Eur J Pharm Sci. 2016 Feb 15;83:143-54. doi: 10.1016/j.ejps.2015.12.023. Epub 2015 Dec 18. Eur J Pharm Sci. 2016. PMID: 26709081
-
Cy5.5-Conjugated anti-epidermal growth factor receptor monoclonal antibody C225.2009 Apr 14 [updated 2009 Jul 22]. In: Molecular Imaging and Contrast Agent Database (MICAD) [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2004–2013. 2009 Apr 14 [updated 2009 Jul 22]. In: Molecular Imaging and Contrast Agent Database (MICAD) [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2004–2013. PMID: 20641731 Free Books & Documents. Review.
-
Synergism of epidermal growth factor receptor-targeted immunotherapy with photodynamic treatment of ovarian cancer in vivo.J Natl Cancer Inst. 2005 Oct 19;97(20):1516-24. doi: 10.1093/jnci/dji314. J Natl Cancer Inst. 2005. PMID: 16234565
-
Photodynamic therapy of squamous cell carcinoma. An evaluation of a new photosensitizing agent, benzoporphyrin derivative and new photoimmunoconjugate.Surg Oncol. 1993;2(3):187-96. doi: 10.1016/0960-7404(93)90006-k. Surg Oncol. 1993. PMID: 8252208
-
Oral cancer: leukoplakia, premalignancy, and squamous cell carcinoma.Dent Clin North Am. 2014 Apr;58(2):315-40. doi: 10.1016/j.cden.2013.12.004. Dent Clin North Am. 2014. PMID: 24655525 Review. No abstract available.
Cited by
-
Targeted Therapy of Cancer Using Photodynamic Therapy in Combination with Multi-faceted Anti-Tumor Modalities.Pharmaceuticals (Basel). 2010 May 14;3(5):1507-1529. doi: 10.3390/ph3051507. Pharmaceuticals (Basel). 2010. PMID: 27713315 Free PMC article. Review.
-
Pulsed Electron Paramagnetic Resonance Imaging: Applications in the Studies of Tumor Physiology.Antioxid Redox Signal. 2018 May 20;28(15):1378-1393. doi: 10.1089/ars.2017.7391. Epub 2018 Jan 9. Antioxid Redox Signal. 2018. PMID: 29130334 Free PMC article. Review.
-
Photoimmunotherapy and irradiance modulation reduce chemotherapy cycles and toxicity in a murine model for ovarian carcinomatosis: perspective and results.Isr J Chem. 2012 Sep;52(8-9):776-787. doi: 10.1002/ijch.201200016. Isr J Chem. 2012. PMID: 23626376 Free PMC article.
-
NIR Photodynamic Destruction of PDAC and HNSCC Nodules Using Triple-Receptor-Targeted Photoimmuno-Nanoconjugates: Targeting Heterogeneity in Cancer.J Clin Med. 2020 Jul 27;9(8):2390. doi: 10.3390/jcm9082390. J Clin Med. 2020. PMID: 32726945 Free PMC article.
-
Expression of epidermal growth factor receptor in the inverted papilloma and squamous cell carcinoma of nasal cavity.Eur Arch Otorhinolaryngol. 2008 Aug;265(8):917-22. doi: 10.1007/s00405-008-0582-3. Epub 2008 Jan 30. Eur Arch Otorhinolaryngol. 2008. PMID: 18231801
References
-
- Speight PM, Morgan PR. The natural history and pathology of oral cancer and precancer. Community Dent. Health. 1993;10(Suppl. 1):31–41. - PubMed
-
- Scully C. Clinical diagnostic methods for the detection of premalignant and early malignant oral lesions. Community Dent. Health. 1993;10(Suppl. 1):43–52. - PubMed
-
- Eisbruch A, Blick M, Lee JS, Sacks PG, Gutterman J. Analysis of the epidermal growth factor receptor gene in fresh human head and neck tumors. Cancer Res. 1987;47:3603–3605. - PubMed
-
- Shirasuna K, Hayashido Y, Sugiyama M, Yoshioka H, Matsuya T. Immunohistochemical localization of epidermal growth factor (EGF) and EGF receptor in human oral mucosa and its malignancy. Virchows Archiv. A Pathol. Anat. Histol. 1991;418:349–353. - PubMed
-
- Ke LD, Adler-Storthz K, Clayman GL, Yung AW, Chen Z. Differential expression of epidermal growth factor receptor in human head and neck cancers. Head Neck. 1998;20:320–327. - PubMed
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials
Miscellaneous