Infectious RNA transcribed in vitro from a cDNA copy of the human coronavirus genome cloned in vaccinia virus
- PMID: 11369870
- DOI: 10.1099/0022-1317-82-6-1273
Infectious RNA transcribed in vitro from a cDNA copy of the human coronavirus genome cloned in vaccinia virus
Abstract
The coronavirus genome is a positive-strand RNA of extraordinary size and complexity. It is composed of approximately 30000 nucleotides and it is the largest known autonomously replicating RNA. It is also remarkable in that more than two-thirds of the genome is devoted to encoding proteins involved in the replication and transcription of viral RNA. Here, a reverse-genetic system is described for the generation of recombinant coronaviruses. This system is based upon the in vitro transcription of infectious RNA from a cDNA copy of the human coronavirus 229E genome that has been cloned and propagated in vaccinia virus. This system is expected to provide new insights into the molecular biology and pathogenesis of coronaviruses and to serve as a paradigm for the genetic analysis of large RNA virus genomes. It also provides a starting point for the development of a new class of eukaryotic, multi-gene RNA vectors that are able to express several proteins simultaneously.
Similar articles
-
Generation of recombinant coronaviruses using vaccinia virus as the cloning vector and stable cell lines containing coronaviral replicon RNAs.Methods Mol Biol. 2008;454:237-54. doi: 10.1007/978-1-59745-181-9_18. Methods Mol Biol. 2008. PMID: 19057873 Free PMC article.
-
Reverse genetics of coronaviruses using vaccinia virus vectors.Curr Top Microbiol Immunol. 2005;287:199-227. doi: 10.1007/3-540-26765-4_7. Curr Top Microbiol Immunol. 2005. PMID: 15609513 Free PMC article. Review.
-
A strategy for the generation of infectious RNAs and autonomously replicating RNAs based on the HCV 229E genome.Adv Exp Med Biol. 1998;440:265-8. doi: 10.1007/978-1-4615-5331-1_34. Adv Exp Med Biol. 1998. PMID: 9782291
-
Recombinant mouse hepatitis virus strain A59 from cloned, full-length cDNA replicates to high titers in vitro and is fully pathogenic in vivo.J Virol. 2005 Mar;79(5):3097-106. doi: 10.1128/JVI.79.5.3097-3106.2005. J Virol. 2005. PMID: 15709029 Free PMC article.
-
Coronavirus genome structure and replication.Curr Top Microbiol Immunol. 2005;287:1-30. doi: 10.1007/3-540-26765-4_1. Curr Top Microbiol Immunol. 2005. PMID: 15609507 Free PMC article. Review.
Cited by
-
Advancements and challenges in mRNA and ribonucleoprotein-based therapies: From delivery systems to clinical applications.Mol Ther Nucleic Acids. 2024 Aug 19;35(3):102313. doi: 10.1016/j.omtn.2024.102313. eCollection 2024 Sep 10. Mol Ther Nucleic Acids. 2024. PMID: 39281702 Free PMC article. Review.
-
Sequence based model using deep neural network and hybrid features for identification of 5-hydroxymethylcytosine modification.Sci Rep. 2024 Apr 20;14(1):9116. doi: 10.1038/s41598-024-59777-y. Sci Rep. 2024. PMID: 38643305
-
An RNA replicon system to investigate promising inhibitors of feline coronavirus.J Virol. 2024 Feb 20;98(2):e0121623. doi: 10.1128/jvi.01216-23. Epub 2024 Jan 18. J Virol. 2024. PMID: 38236006 Free PMC article.
-
Rapid cloning-free mutagenesis of new SARS-CoV-2 variants using a novel reverse genetics platform.Elife. 2023 Nov 21;12:RP89035. doi: 10.7554/eLife.89035. Elife. 2023. PMID: 37988285 Free PMC article.
-
New Short RNA Motifs Potentially Relevant in the SARS-CoV-2 Genome.Curr Genomics. 2023 Feb 14;23(6):424-440. doi: 10.2174/1389202924666230202152351. Curr Genomics. 2023. PMID: 37920558 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
